Objective: To Compare the changes of different rat models of liver-stagnation syndrome

spleen-deficiency syndrome and both liver-stagnation and spleen-deficiency syndrome with thyroid function and study on the effective of application soothing liver Qi and tonifying spleen Qi formula to those rat models. Method: The male wistar rats were randomly assigned into the normal group (group 1)

the liver-stagnation group (group 2)

the spleen-deficiency group (group 3)

the liver-stagnation and spleen-deficiency group (group 4)

the drug treatment to liver-stagnation group (group 5)

the drug treatment to spleen-deficiency group (group 6) and the drug treatment to liver-stagnation and spleen-deficiency group (group 7)with ten rats for each group. The models were established by chronic restraint method in group 2 and 5

by excess fatigue plus out of constant diet method in group 3 and 6

by chronic restraint-fatigue-diet disorder method in group 4 and 7 for 4 weeks. On the 15th day

rats of group 5

6 and 7 had been fed with medicine of Chaishu Sijunzi decoction 3.57 g·kg-1 for two weeks. The ones of the normal group and model group 2

3 and 4 were fed with equivalent physiological saline for two weeks. On the 29th day

all rats of serum levels of 3

5

3'-triiodothyronine (T3)

thyroxin

3

5

3

5'-tetraiodothyronine (T4)

thyroid stimulating hormone (TSH) and both of serum and hypothalamus tyrotropin releasing hormone (TRH) were detected. Result: Compared with group 1

serum T3

T4 and TRH decreased significantly and serum TSH increased significantly in group 2

3 and 4 (P<0.05)

hypothalamus TRH decreased significantly in group 3 and 4 (P<0.05)

hypothalamus TRH had no significantly changing in group 2.Compared with group 2

serum T4 increased significantly and serum TSH decreased significantly in group 5 (P<0.05). Compared with group 3

serum T3 increased significantly and serum TSH decreased significantly in group 6 (P<0.05) and hypothalamus TRH had increasing tendency in group 6.Compared with group 4

serum T3 and hypothalamus TRH increased significantly and serum TSH decreased significantly in group 7 (P<0.05) and serum T4 had increasing tendency in group 6. Conclusion: The function of hypothalamus-ptuitary-thyroidal axis was abnormal at different degrees in rat models of liver-stagnation syndrome

spleen-deficiency syndrome and both liver-stagnation and spleen-deficiency syndrome with hypothyroidism. Liver-stagnation syndrome was mainly involved the function of releasing impairment with hypothalamus TRH. Both of spleen-deficiency and liver-stagnation and spleen-deficiency syndromes were not only involved in releasing impairment

but also involved in synthesis restraining with hypothalamus TRH. The formula of soothing liver Qi and tonifying spleen Qi can improved the function of thyroid gland and thyroidal axis to those rat models;however the best improvement of comprehensive regulating function was to liver-stagnation and spleen-deficiency syndrome model.