Objective: To observe the effects of jujuboside(JBSD)on glutamic acid(Glu)

γ-aminobutyric acid(GABA) and γ-aminobutyric acid A receptor (GABAAR) expression in the brain of syndrome insomnia old rats. Method: Wistar rats were randomly divided into 5 groups:blank control

syndrome model group

positive medicine group

high and low-dose JBSD treatment group. Multiple factors model were established with subacute senescence induced by D-galactose

Yin and blood-deficiency induced by cyclophosphamide and hydrocortisonum

and the rapid eye movement(REM) sleep deprivation(SD) induced by maded-self multiple platform method (MMPM).After three weeks of administration

the levels of amino acid neurotransmitters Glu and GABA in cortex and hippocampus were detected by HPLC

and GABAAR expression were detected by immunohistochemical staining and retrovirus-polymerase chain reaction (RT-PCR). After intervention of jujuboside

those indicators were studied. Result: Compared to environmental control group

in model group the content of Glu and GABA and the ratio of them increased in cortex and hypothalamus (P<0.01

P<0.05)

GABAAR immunochemistry absorbance was rose significantly (P<0.01) in cortex and hippocampus

and GABAAR mRNA expression showed higher significantly (P<0.01) in cortex.While the indicators tended to the normal range after the treatment of JBSD (P<0.01

P<0.05). Conclusion: JBSD can reduce the toxic effects of amino acids in brain

and downregulating GABAAR expression in the rat brain may be one of the mechanisms in treatment of the syndrome insomnia.