ZHAO Yi, LI Bing-tao, LAI Xiao-dong, et al. Dose-effect Relationship of Gegen Qinlian Decoction in Treating the Ulcerative Colitis Rats[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(4): 131-134.
DOI:
ZHAO Yi, LI Bing-tao, LAI Xiao-dong, et al. Dose-effect Relationship of Gegen Qinlian Decoction in Treating the Ulcerative Colitis Rats[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(4): 131-134. DOI: 10.11653/syfj2014040131.
Dose-effect Relationship of Gegen Qinlian Decoction in Treating the Ulcerative Colitis Rats
Objective: To explore the dose-effect relationship and‘dose threshold’parameter of Gegen Qinlian decoction in treating the picrylsulfonic acid solution (TNBS) indeced-ulcerative colitis rats. Method: The experimental ulcerative colitis model induced by TNBS/ethanol
except for the normal group
then divided into model group
sulfasalazine group
and Gegen Qinlian decoction 1.65
4.95
8.25
11.55
14.85
18.15
21.45
24.75
28.05 g·kg-1
administered once a day for 5 days.then measured and calculated the disease activity index (DAI) index
and detected the myeloperoxidase(MPO)
malondialdehyde(MDA)
superoxide dismutase(SOD levels in tissue homogenate.Through used the GraphPad Prism 6 software to establish the dose-response and calculate the dose-related parameters. Result: By the comprehensive analysis of experimental data
the dose-effect relationship of MPO appeared the‘S’shape
and the dose threshold parameters are as follows:D20-D80 15.39-17.11 g·kg-1
median dose:D50 16.25 g·kg-1
the threshold dose:D20 15.39 g·kg-1. Conclusion: Gegen Qinlian decoction showed dose-response curves as‘S’shape in treatment of ulcerative colitis rats
suggesting a dose-dependent range is between 15.39 g·kg-1 to 17.11 g·kg-1.
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