Investigation of Extraction and Purification Process for Total Saponins in Trigonellae Semen

HEI Jing; CHEN Zhi; LEI Ya-ya; WANG Wen-ping; SUI Hong

doi:10.11653/syfj2014050011

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Investigation of Extraction and Purification Process for Total Saponins in Trigonellae Semen

更新时间：2024-01-04

- Investigation of Extraction and Purification Process for Total Saponins in Trigonellae Semen
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 5, Pages: 11-13(2014)
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- DOI：10.11653/syfj2014050011
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- Published：2014
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HEI Jing, CHEN Zhi, LEI Ya-ya, et al. Investigation of Extraction and Purification Process for Total Saponins in Trigonellae Semen[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(5): 11-13.
DOI：

HEI Jing, CHEN Zhi, LEI Ya-ya, et al. Investigation of Extraction and Purification Process for Total Saponins in Trigonellae Semen[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(5): 11-13. DOI： 10.11653/syfj2014050011.

摘要

目的：制备高纯度的胡芦巴总皂苷。方法：采用乙醇回流法提取胡芦巴总皂苷，依次加正己烷、三氯甲烷、乙酸乙酯、正丁醇进行系统溶剂萃取，三氯甲烷有效部位经HPD-400型大孔吸附树脂富集纯化。采用显色反应和TLC鉴别总皂苷，运用UV测定胡芦巴总皂苷含量。结果：三氯甲烷萃取部位中总皂苷纯度40%，得率6.28%；水洗脱液中胡芦巴总皂苷纯度80.5%，得率0.32%。结论：优选的提取纯化工艺快速简便，为胡芦巴总皂苷的药效学评价奠定基础。

Abstract

Objective: To prepare total saponins of Trigonellae Semen with high purity. Method: Total saponins of Trigonellae Semen was extracted by ethanol refluxing method

then extract was dealt with different solvents systematically

including n-hexane

chloroform

ethyl acetate

n-buthanol.Chloroform effective part was purified through HPD-400 macroreticular resin.Total saponins in each steps and fractions was identified by chromogenic reaction and TLC

contents of total saponins were determined by ultraviolet spectroscopy. Result: Purity of total saponins in chloroform solvent extraction was 40% with yield of 6.28%;Purity of total saponins in water eluent was 80.5% with yield of 0.32%. Conclusion: This optimized process was convenient and efficient

it could lay foundation for pharmacodynamic study.

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Related Institution

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