Transport Characteristics of -Cyperone in Caco-2 Cell Model by LC-MS

HU Lv-jiang; GUO Hui-ling; HU Zhi-fang; YAN Bai-yi; ZHAO Xiao-juan; XIAO Xiao-mei

doi:10.11653/syfj2014050035

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Transport Characteristics of -Cyperone in Caco-2 Cell Model by LC-MS

更新时间：2024-01-04

- Transport Characteristics of -Cyperone in Caco-2 Cell Model by LC-MS
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 5, Pages: 35-38(2014)
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- DOI：10.11653/syfj2014050035
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- Published：2014
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HU Lv-jiang, GUO Hui-ling, HU Zhi-fang, et al. Transport Characteristics of -Cyperone in Caco-2 Cell Model by LC-MS[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(5): 35-38.
DOI：

HU Lv-jiang, GUO Hui-ling, HU Zhi-fang, et al. Transport Characteristics of -Cyperone in Caco-2 Cell Model by LC-MS[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(5): 35-38. DOI： 10.11653/syfj2014050035.

摘要

目的：考察α-香附酮在Caco-2细胞模型中转运机制。方法：建立Caco-2细胞模型，考察体积分数和时间对α-香附酮双向转运过程的影响。采用LC-MS测定α-香附酮含量，流动相甲醇（A）-水（B）梯度洗脱（0~5 min，30%~70%A；5~10 min，70%~100%A；15~20 min，30%A），流速0.25 mL·min-1，计算不同条件下表观渗透系数（Papp）。结果：α-香附酮在2 h内转运量与时间、体积分数呈正相关。不同体积分数α-香附酮从BL侧到AP侧的Papp与AP侧到BL侧的Papp比值均在0.5~1.5。结论：α-香附酮在Caco-2细胞模型中转运特性为被动扩散转运方式。

Abstract

Objective: To investigate transport mechanisms of α-cyperone in Caco-2 cell model by LC-MS. Method: Caco-2 cell model was established

effects of volume fraction and time on bidirectional transport process of α-cyperone were investigated.LC-MS was adopted to determine the content of α-cyperone with mobile phase of methanol(A)-water(B)(0-5 min

30%-70%A;5-10 min

70%-100%A;15-20 min

30%A) and a flow rate of 0.25 mL·min-1

values of apparent permeability coefficient(Papp) under different conditions were calculated. Result: Both transport amounts of AP-to-BL and BL-to-AP increased with increasing of volume fraction and time within 2 h.Papp kept 0.5-1.5 at different volume fractions of α-cyperone. Conclusion: α-Cyperone could be transported through intestinal mucosa via a passive diffusion mechanism.

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