Determination of Three Naphthopyrone Glycosides in  by HPLC

XU Yi-long; TANG Li-ying; ZHOU Xi-dan; ZHOU Guo-hong; WANG Zhu-ju

doi:10.11653/syfj2014050054

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Determination of Three Naphthopyrone Glycosides in by HPLC

更新时间：2024-01-04

- Determination of Three Naphthopyrone Glycosides in by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 5, Pages: 54-56(2014)
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- DOI：10.11653/syfj2014050054
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- Published：2014
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XU Yi-long, TANG Li-ying, ZHOU Xi-dan, et al. Determination of Three Naphthopyrone Glycosides in by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(5): 54-56.
DOI：

XU Yi-long, TANG Li-ying, ZHOU Xi-dan, et al. Determination of Three Naphthopyrone Glycosides in by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(5): 54-56. DOI： 10.11653/syfj2014050054.

摘要

目的：建立HPLC同时测定决明子中红镰霉素龙胆二糖苷、决明子苷、决明子苷C含量的方法。方法：采用HPLC，Dionex Acclaim C18柱（4.6 mm×250 mm，5 μm），流动相乙腈-四氢呋喃-1%冰醋酸（17：2：81），流速1 mL·min-1，柱温30 ℃，检测波长278 nm。结果：红镰霉素龙胆二糖苷、决明子苷、决明子苷C的进样量分别在0.218~1.090，0.188~0.940，0.200~1.000 μg和峰面积呈良好的线性关系，回归方程分别为Y=54.017X-0.344 6 （r=0.999 9），Y=78.63X-0.354（r=0.999 9），Y=74.098X-0.433 5 （r=0.999 9）；平均加样回收率分别为98.43%（RSD 1.75%），101.89%（RSD 1.10%），102.46%（RSD 1.15%）。结论：该方法快速、灵敏、准确、可靠、重复性好，可用于中药决明子药材的质量控制。

Abstract

Objective: To establish a high performance liquid chromatographic method (HPLC) for simultaneous determination of rubrofusarin gentiobioside

casside

cassiaside C in Cassia obtusifolia. Method: The HPLC with Dionex Acclaim C18(4.6 mm×250 mm

5 μm) column was used

acetonitrile-THF-1% acetic acid (17 ：2 ：81) was used as a mobile phase

with flow rate of 1 mL·min-1

column temperature at 30 ℃ and detection wavelength at 278 nm. Result: Rubrofusarin gentiobioside

casside

cassiaside C showed good linearity (r=0.999 9) in the range of 0.218-1.09

0.188-0.94

0.200-1.000 μg

regressive equation were Y=54.017X-0.344 6 (r=0.999 9)

Y=78.63X-0.354(r=0.999 9)

Y=74.098X-0.433 5 (r=0.999 9)respectively;the average recoveries of the method were 98.43%

101.89%

102.46%

RSD were 1.75%

1.10%

1.15%. Conclusion: The method was proved to be simple

rapid

sensitive

precise

reliable and repeatable. It can be applied to the quality control of Semen Cassia.

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Related Institution

School of Pharmaceutical Sciences， Changchun University of Chinese Medicine

Jilin Ginseng Academy，Changchun University of Chinese Medicine

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