ZHANG De-quan, MA A-li, YANG Yong-shou, et al. Content Determination of Trihydroxybenzoic Acid and Paeonol in from Different Producing Area by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(5): 57-60.
DOI:
ZHANG De-quan, MA A-li, YANG Yong-shou, et al. Content Determination of Trihydroxybenzoic Acid and Paeonol in from Different Producing Area by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(5): 57-60. DOI: 10.11653/syfj2014050057.
Content Determination of Trihydroxybenzoic Acid and Paeonol in from Different Producing Area by HPLC
Objective: To establish determined method of the content of trihydroxybenzoic acid and paeonol in Paeonia delavayi
and compare the content in samples from different areas and samples from different parts. Method: The samples were separated by a ZORBOX SB-C18 (4.6 mm×250 mm
5 μm) using acetonitrile-0.1% methane acid with water gradient system as mobile phase. The flow rate was 1.0 mL·min-1
and the detection wavelength for fingerprinting was 275 nm with 30 ℃ for column temperature. Result: There were well linear relationships for trihydroxybenzoic acid and paeonol in range of 0.204 0-2.040 μg and 0.204 0-2.040 μg. And average recovery rates for them (n=6) were 101.81% and 97.80%. Conclusion: The method could be used for quality control to medicinal materials of P. delavayi. Contents of trihydroxybenzoic acid and paeonol were significantly different for medicinal materials collected from different areas.
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Related Author
Xia-xia FENG
Hong YI
Li-hua YAN
Wen-wen ZHANG
Yu YAN
Qin SI
Xiao-qian LIU
Zhi-min WANG
Related Institution
College of Pharmacy, Shaanxi University of Chinese Medicine
National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine, Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences
Chengde Jingfukang Pharmaceutical Group Co.Ltd,New Excipients of Traditional Chinese MedicineEngineering Research Center of Hebei Province