Objective: To establish the HPLC fingerprint of the extract of physical defatted roasted Raphani Semen. Method: Yilit Hypersil C18 (4.6 mm×250 mm

5 μm) was used;flow rate was 1.0 mL·min-1;column temperature was kept at 30 ℃;the mobile phase was composed of acetonitrile (A)-0.1%H3PO4(B) with gradient elution. The concentrations of solvent A were 5%

30%

70% and 88% at 0

28

30

51 min respectively. Result: The HPLC fingerprint of the extract of physical defatted roasted Raphani Semen was set up

showing 10 characteristic peaks. The HPLC fingerprint similarity of the extract of physical defatted roasted Raphani Semen from ten different growing areas is greater than 0.96. Conclusion: This method is stable

accurate with a good reproducibility and can be used to evaluate the qualitative identification of the extract of physical defatted roasted Raphani Semen.