Simultaneous Determination of Paeoniflorin,Baicalin and Berberine in the Mixed Decoction of Huanglian Ejiaotang by HPLC

ZHANG Zhen-ling; FENG Sheng-nan; JIA Li-li

doi:10.11653/syfj2014050075

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Simultaneous Determination of Paeoniflorin,Baicalin and Berberine in the Mixed Decoction of Huanglian Ejiaotang by HPLC

更新时间：2024-01-04

- Simultaneous Determination of Paeoniflorin,Baicalin and Berberine in the Mixed Decoction of Huanglian Ejiaotang by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 5, Pages: 75-77(2014)
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- DOI：10.11653/syfj2014050075
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- Published：2014
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ZHANG Zhen-ling, FENG Sheng-nan, JIA Li-li. Simultaneous Determination of Paeoniflorin,Baicalin and Berberine in the Mixed Decoction of Huanglian Ejiaotang by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(5): 75-77.
DOI：

ZHANG Zhen-ling, FENG Sheng-nan, JIA Li-li. Simultaneous Determination of Paeoniflorin,Baicalin and Berberine in the Mixed Decoction of Huanglian Ejiaotang by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(5): 75-77. DOI： 10.11653/syfj2014050075.

摘要

目的：建立高效液相色谱法同时测定黄连阿胶汤中芍药苷、黄芩苷、小檗碱3种成分的含量。方法：采用Agilent TC-C18色谱柱（4.6 mm×250 mm），流动相乙腈-0.1%磷酸溶液梯度洗脱，流速1.0 mL·min-1，检测波长240 nm，柱温25 ℃。结果：黄连阿胶汤中芍药苷、黄芩苷、小檗碱平均质量分数分别为1.64%，7.51%，3.30%。结论：该法操作简便、准确且精密度和重复性良好，可用于黄连阿胶汤的质量控制。

Abstract

Objective: A simple and rapid HPLC method was established for simultaneously determining of paeoniflorin

baicalin and berberine in the mixed decoction of Huanglian Ejiaotang. Method: Agilent TC-C18(4.6 mm×250 mm)chromatographic column

acetonitrile-0.1% phosphoric acid solution gradient elution were applied. Analyses were performed at 35 ℃ with a flowrate of 1.0 mL·min-1 and UV detection at 240 nm

Result: The content of paeoniflorin

baicalin and berberine were 1.64%

7.51%

3.30% respectively. Conclusion: The developed method can be applied to the intrinsic quality control of Huanglian Ejiaotang.

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