Effect and Mechanism of Chemical Constituents from  on Inhibiting Xanthinen Oxidase

QI Wan-hu; JIANG Qi-zhou; JIANG Jian-qin

doi:10.11653/syfj2014050141

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Effect and Mechanism of Chemical Constituents from on Inhibiting Xanthinen Oxidase

更新时间：2024-01-04

- Effect and Mechanism of Chemical Constituents from on Inhibiting Xanthinen Oxidase
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 5, Pages: 141-144(2014)
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- DOI：10.11653/syfj2014050141
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- Published：2014
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QI Wan-hu, JIANG Qi-zhou, JIANG Jian-qin. Effect and Mechanism of Chemical Constituents from on Inhibiting Xanthinen Oxidase[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(5): 141-144.
DOI：

QI Wan-hu, JIANG Qi-zhou, JIANG Jian-qin. Effect and Mechanism of Chemical Constituents from on Inhibiting Xanthinen Oxidase[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(5): 141-144. DOI： 10.11653/syfj2014050141.

摘要

目的：黄瑞香中化学成分对黄嘌呤氧化酶活性的影响及其机制探讨。方法：以黄嘌呤为底物，别嘌呤醇为阳性药，采用分光光度法测定黄瑞香中的化学成分木犀草素，5，7，3’-三羟基-4’-甲氧基黄酮daphnodorin A（3），瑞香苷对黄嘌呤氧化酶活性的影响，并进一步对上述化合物进行酶抑制类型考察。结果：黄瑞香中化学成分木犀草素，5，7，3’-三羟基-4’-甲氧基黄酮，daphnodorin A，瑞香苷对黄嘌呤氧化酶有明显的抑制作用，且5，7，3’-三羟基-4’-甲氧基黄酮为非竞争性抑制，木犀草素、瑞香苷为竞争性抑制黄嘌呤氧化酶。结论：黄瑞香中的黄酮类和香豆素类成分能够较好的抑制黄嘌呤氧化酶，为黄瑞香治疗痛风奠定了一定的理论基础。

Abstract

Objective: To study the inhibitory effect and mechanism of chemical constituents from Daphne giraldii on xanthine oxidase activity. Method: The ultraviolet spectrophotometry was adopted

xanthine as substrate and allopurinol as positive control drug

to measure xanthine oxidase activity affected by four compounds:luteolin(1)

3'-trihydroxy-4'-methoxy-flavone(2)

daphnodorin A(3)

daphnin(4) which were isolated from D. giraldii

and further the type of inhibition through enzyme kinetic was exploreds. Result: The compounds had obvious inhibiting effect on xanthine oxidase

among which 5

3'-trihydroxy-4'-methoxy-flavone(2)was a noncompetitive inhibitor while luteolin(1)

daphnodorin A(3) daphnin(4)were competitive inhibitors. Conclusion: Flavonoids and coumarins in D. giraldii can effectively inhibit xanthine oxidase activity

which can provide theoretical basis for the clinical usage of D. giraldii in treatment of gout.

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Related Institution

School of Pharmacy，State Key Laboratory of Southwest Chinese Medicine Resources， Chengdu University of Traditional Chinese Medicine

College of Pharmacy， Liaoning University of Traditional Chinese Medicine （TCM）

Liaoning Multi-dimensional Analysis of TCM Technical Innovation Center

China Resources Sanjiu Medical & Pharmaceutical Co. Ltd.

The Second People's Hospital of Dalian

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