NI Bin, LI Xiang, LUO Yun, et al. Preparation of Curcumin-Loaded Ophthalmic Liposomes and Investigation of Its Lenses Antioxidant Efficacy[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(6): 5-9.
DOI:
NI Bin, LI Xiang, LUO Yun, et al. Preparation of Curcumin-Loaded Ophthalmic Liposomes and Investigation of Its Lenses Antioxidant Efficacy[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(6): 5-9. DOI: 10.11653/syfj2014060005.
Preparation of Curcumin-Loaded Ophthalmic Liposomes and Investigation of Its Lenses Antioxidant Efficacy
Objective: To prepare N-trimethyl chitosan(TMC)-coated liposomes loaded with curcumin (Cur-TCL) and investigate its in vivo antioxidant effect on selenite-induced cataract lenses. Method: Cur-TCL were prepared by ethanol injection method and formulation was optimized by central composite design-response surface methodology;Membrane filter
transmission electron microscope
dynamic light scattering and electrophoretic light scattering were employed to determined physicochemical parameters of Cur-TCL;Selenite cataract model in rats was established
inhibition of Cur-TCL on oxidation process in selenite cataract formation was investigated. Result: Optimal formulation was as following:phospholipids concentration 4%
cholesterol-phospholipid(1:5.44)
curcumin 1.41 mg and the concentration of TMC solution 0.5%.Cur-TCL was spherical-shaped with increasing of mean particle sizes
signigicantly improving of Zeta potential and drug encapsulation efficiency basically unchanged(92.5±0.35)% after coating liposomes with TMC.Compared with uncoated liposomes
SOD activity was elevated by 30.3%
molar concentration of MDA decreased by 52.4% and the content of GSH increased 1.29-fold. Conclusion: Cur-TCL could significantly delay lenses oxidation process of selenite cataract.This work was useful for further research on application of Cur-TCL for cataract therapy.