WU Zhen-feng, WANG Ya-qi, YANG Ming, et al. Investigation of Stability and Optimization of Vacuum Extraction Technology for Active Constituents in Gardeniae Fructus[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(6): 10-14.
DOI:
WU Zhen-feng, WANG Ya-qi, YANG Ming, et al. Investigation of Stability and Optimization of Vacuum Extraction Technology for Active Constituents in Gardeniae Fructus[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(6): 10-14. DOI: 10.11653/syfj2014060010.
Investigation of Stability and Optimization of Vacuum Extraction Technology for Active Constituents in Gardeniae Fructus
Objective: To investigate stability of geniposide and crocin-I in water extract of Gardeniae Fructus and optimize their vacuum extraction process. Method: Stability of geniposide and crocin-I in water extract was studied by classical constant temperature acceleration test.With extract yield
contents of geniposide and crocin-I as dependent variables
solid-liquid ratio
extraction temperature and time as independent variables
Box-Behnken design-response surface methodology was optimize vacuum extraction technology. Result: Optimal process parameters was as follows:extraction temperature 45 ℃
extraction time 60 min
ratio of liquid to solid 14:1;Under these conditions
extract yield
contents of geniposide and crocin Ⅰ were 28.24%(0.96%)
3.51%(1.44%) and 1.21%(2.21%)
respectively. Conclusion: Vacuum extraction method was suitable for Chinese herbals which contained heat-sensitive components.This optimized process was reasonable and practicable
which could provide useful reference for comprehensive utilization of Gardeniae Fructus.