Optimization of Purification Technology for Total Flavonoids from  by Polyamide Resin

ZHANG Peng-wei; SU Wen-qin; LIU Xiao; LIU Yong

doi:10.11653/syfj2014060023

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Optimization of Purification Technology for Total Flavonoids from by Polyamide Resin

更新时间：2024-01-04

- Optimization of Purification Technology for Total Flavonoids from by Polyamide Resin
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 6, Pages: 23-25(2014)
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- DOI：10.11653/syfj2014060023
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- Published：2014
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ZHANG Peng-wei, SU Wen-qin, LIU Xiao, et al. Optimization of Purification Technology for Total Flavonoids from by Polyamide Resin[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(6): 23-25.
DOI：

ZHANG Peng-wei, SU Wen-qin, LIU Xiao, et al. Optimization of Purification Technology for Total Flavonoids from by Polyamide Resin[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(6): 23-25. DOI： 10.11653/syfj2014060023.

摘要

目的：优选聚酰胺树脂分离纯化裸花紫珠总黄酮的工艺条件。方法：以总黄酮含量为指标，利用静态-动态吸附-洗脱试验考察聚酰胺树脂纯化总黄酮的行为，通过单因素试验考察饱和吸附量、pH、洗脱溶剂用量等工艺参数。结果：最佳精制工艺参数为上样液质量浓度0.073 g·mL-1，上样量2 BV，上样液pH 3.0，加水3 BV洗脱杂质，用80%乙醇3 BV洗脱，收集乙醇洗脱液；总黄酮纯度由5.6%提高至42.3%，平均保留率89.6%（RSD 3.56%）。结论：采用聚酰胺树脂纯化裸花紫珠总黄酮的效果良好，为裸花紫珠新制剂的开发提供实验依据。

Abstract

Objective: To optimize purification technology of total flavonoids from Callicarpa nudiflora by polyamide resin. Method: With the content of total flavonoids as index

adsorption and elution behavior of polyamide resin for total flavonoids was investigated by stastic-dynamic adsorption-elution test

single factor tests were adopted to optimize purification technology for total flavonoids taking saturated adsorption

elution volume and other parameters. Result: Optimum purification technology parameters were as follows:the concentration of sample solution 0.073 g·mL-1

sample volume of 2 BV

pH of sample solution 3.0

eluted impurities with 3 BV water

then eluted with 3 BV of 80% ethanol;Purity of total flavonoids was up to 42.3% from 5.6%

average retention rate was 89.6%(RSD 3.56%). Conclusion: Purification effect of total flavonoids from C. nudiflora by polyamide resin was feasible

and this study could provide experimental basis for development of new formulations from C. nudiflora.

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