Optimization of Extraction Technology for  by Central Composite Design-Response Surface Methodology

ZHAO Yong-heng; ZHOU Yi-sheng; WANG Song; LIN Li-wei

doi:10.11653/syfj2014060036

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Optimization of Extraction Technology for by Central Composite Design-Response Surface Methodology

更新时间：2024-01-04

- Optimization of Extraction Technology for by Central Composite Design-Response Surface Methodology
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 6, Pages: 36-39(2014)
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- DOI：10.11653/syfj2014060036
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- Published：2014
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ZHAO Yong-heng, ZHOU Yi-sheng, WANG Song, et al. Optimization of Extraction Technology for by Central Composite Design-Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(6): 36-39.
DOI：

ZHAO Yong-heng, ZHOU Yi-sheng, WANG Song, et al. Optimization of Extraction Technology for by Central Composite Design-Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(6): 36-39. DOI： 10.11653/syfj2014060036.

摘要

目的：优选龙须藤的提取工艺。方法：以乙醇体积分数、液料比、提取时间为自变量，浸膏得率、3’，4’，5’，5，7-五甲氧基黄酮（黄酮1）和3’，4’-亚甲二氧基-5’，5，6，7-四甲氧基黄酮（黄酮2）含量的总评“归一值”为因变量，通过Design-Expert 7.0.0软件对自变量各水平进行多元线性回归和二项式拟合，利用效应面法优选提取工艺并进行预测分析。采用HPLC测定黄酮1和黄酮2含量，色谱条件为流动相乙腈（A）-水（B）洗脱梯度（0~20 min，30%~40% A；20~36 min，40%~48% A），检测波长324 nm。结果：二项式拟合的复合相关系数较高，总评“归一值”达0.995 6；最佳提取工艺为加14倍量45%乙醇提取2次，每次116 min；黄酮1和黄酮2的质量分数依次为0.046%，0.027%，浸膏得率21.21%。结论：利用星点设计-效应面法优化的龙须藤提取工艺稳定简便，具有良好的预测性。

Abstract

Objective: To optimize extraction process of Bauhinia championi. Method: Taking ethanol concentration

ratio of material to liquid and reflux time as independent variables

overall desirability of dry extract yield

contents of 3'

7-pentamethoxyflavone(flavonoids 1) and 3'

4'-methylenedioxy-5'

7-tetramethoxyflavone(flavonoids 2) as dependent variable

data of overall desirabilities were fitted to a second-order polynomial equation and regressed by multiple linear

response surface methodology was used to optimize extraction technology and predictive analyze.HPLC was adopted to determine contents of flavonoids 1 and flavonoids 2 with mobile phase of acetonitrile(A)-water(B) gradient elution(0-20 min

30%-40% A;20-36 min

40%-48% A) and detection wavelength of 324 nm. Result: Complex correlation coefficient of binomial fitting was higher with overall desirability of 0.995 6;Optimal extraction technology was as following:extracted 2 times with 14-fold the amount of 45% ethanol

116 min per time;The mass fractions of flavonoids 1 and flavonoids 2 were 0.046% and 0.027%

dry extract yield was 21.21%. Conclusion: Central composite design-response surface methodology was convenient and highly predictive for optimizing extraction technology of B. championi.

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