Objective: To establish methods for determination of astragaloside Ⅳ and hydroxysafflor yellow A in Luhong granules. Method: The content of astragaloside Ⅳ was determined by HPLC-ELSD

chromatography was performed on a Kromasil C18 column(4.6 mm×250 mm

5 μm) at 25 ℃

mobile phase composition was acetonitrile-water(34:66)

flow rate was at 1.0 mL·min-1;An ELSD was used as detector with gas flow rate of 1.5 mL·min-1

nebulization temperature at 40 ℃ and evaporative light temperature at 90 ℃.HPLC-VWD was adopted to determine the content of hydroxysafflor yellow A with mobile phase of methanol-acetonitrile-0.2% phosphoric acid (35:2:63)

column temperature at 30 ℃

flow rate of 1.0 mL·min-1

detection wavelength at 403 nm. Result: Regression equation of astragaloside Ⅳ was Y=1.692 5X+5.832 2(R2=0.999 1) with linear range of 0.165 2-10.58 μg and average recovery of 98.56%(RSD 1.99%);Regression equation of hydroxysafflor yellow A was Y=0.032 5X+0.444 9(R2=1.000) with linear range of 0.021-1.34 μg and average recovery of 101.84%(RSD 2.54%);The mass fraction of them in samples were 0.333

1.803 mg·g-1

respectively. Conclusion: These methods were simple and feasible with good reproducibility

which were suitable for quality control of Luhong granules.