WANG Man-yuan, ZHANG Dong, LI Zhao-xia, et al. Targeted Separation and Determination of Arteannuic Acid in Growing in Beijing[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(6): 48-51.
DOI:
WANG Man-yuan, ZHANG Dong, LI Zhao-xia, et al. Targeted Separation and Determination of Arteannuic Acid in Growing in Beijing[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(6): 48-51. DOI: 10.11653/syfj2014060048.
Targeted Separation and Determination of Arteannuic Acid in Growing in Beijing
Objective: To establish targeted separation and determination of the semi-synthesis precursor compound of artemisinin
arteannuic acid
in Artemisia annua growing in Beijing. Method: Arteannuic acid in plant was determined by HPLC with ODS column. The mobile phase was methanol-0.05% HOAc (85:15) solution at a flow rate of 0.8 mL·min-1. The UV detection wavelength was 220 nm. Arteannuic acid was extracted by maceration using cold 95% ethanol and purified by silica gel chromatography as well as recrystallization. Result: Arteannuic acid in sample solution was well separated. The average recovery was 98.39% with RSD of 0.81%(n=6) and the linear range was 0.11-2.76 μg. The content range of arteannuic acid was 0.36%-0.58% in A. annua growing in Beijing. The purity of arteannuic acid isolated follow the given process was higher than 98%. Conclusion: The targeted separation and determination method were simple
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