Determination of Content of Galuteolin in Different Varieties of  Flos and  Folium by HPLC

ZHAO Jin-juan; GUAN Ren-wei; LU Jun-xian; WANG Meng; DU Xiao-xuan; LIN Hui-bin; LIN Jian-qiang

doi:10.11653/syfj2014060103

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Determination of Content of Galuteolin in Different Varieties of Flos and Folium by HPLC

更新时间：2024-01-04

- Determination of Content of Galuteolin in Different Varieties of Flos and Folium by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 6, Pages: 103-106(2014)
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- DOI：10.11653/syfj2014060103
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- Published：2014
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ZHAO Jin-juan, GUAN Ren-wei, LU Jun-xian, et al. Determination of Content of Galuteolin in Different Varieties of Flos and Folium by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(6): 103-106.
DOI：

ZHAO Jin-juan, GUAN Ren-wei, LU Jun-xian, et al. Determination of Content of Galuteolin in Different Varieties of Flos and Folium by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(6): 103-106. DOI： 10.11653/syfj2014060103.

摘要

目的：比较不同品种之间金银花及叶中黄酮类成分木犀草苷含量的差异。方法：采用Diamonsil-C18色谱柱（150 mm×4.6 mm，5 μm），乙腈-0.5%冰醋酸溶液为流动相梯度洗脱，流速1.0 mL·min-1，柱温35 ℃，检测波长350 nm。结果：木犀草苷在0.015 6~0.5 g·L-1呈良好线性关系，平均回收率为96.88%。结论：1~10号金银花样品中木犀草苷的含量在0.08%~0.22%之间，均高于《中国药典》规定的0.050%，其中亚特良种金银花（意大利种质）及九丰一号含量较高。红金银花中木犀草苷含量为0.11%，符合《中国药典》该指标要求。金银花叶中木犀草苷的含量分别为0.75%，0.76%，明显高于花中木犀草苷的含量。

Abstract

Objective: To compare differences of the content of galuteolin between different varieties of Lonicerae japonicae Flos and L. Japonicae Folium. Method: The separation was performed on a Diamonsil-C18 column(150 mm×4.6 mm

5 μm)

acetonitrile-0.5% acetic acid was used as the mobile phase with gradient elution

the flowrate was 1.0 mL·min-1

column temperature was 35 ℃

the detection wavelength was at 350 nm. Result: The linear range (n=6)was 0.015 625-0.5 g·L-1 (R2=1) respectively. The averagerecoveries of galuteolin was 96.88% respectively. Conclusion: The content of galuteolin in from 1 to 10 sample is between 0.08% and 0.22%

which is higher than 0.050% of requirement of ChP.The content of galuteolin in ya te improved variety L. japonicae Flos (introduced from Italy)and jiu feng yi hao is higher than others samples. The content of galuteolin in L. japonica var.chinensis is 0.11%

which meets the medicinal requirements. The content of galuteolin in L. japonicae Folium is between 0.75% and 0.76%

which is apparently higher than it in L. japonicae Flos.

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