Influence of Maixuekang Capsule on Lipid Metabolism and Expression of Lectin Like Oxidized Low Density Lipoprotein Receptor-1 in Early Atherosclerosis Rats

HU Yao-hong; YANG Qiao; GAO Li-juan

doi:10.11653/syfj2014060157

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Influence of Maixuekang Capsule on Lipid Metabolism and Expression of Lectin Like Oxidized Low Density Lipoprotein Receptor-1 in Early Atherosclerosis Rats

更新时间：2024-01-04

- Influence of Maixuekang Capsule on Lipid Metabolism and Expression of Lectin Like Oxidized Low Density Lipoprotein Receptor-1 in Early Atherosclerosis Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 6, Pages: 157-161(2014)
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- DOI：10.11653/syfj2014060157
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- Published：2014
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HU Yao-hong, YANG Qiao, GAO Li-juan. Influence of Maixuekang Capsule on Lipid Metabolism and Expression of Lectin Like Oxidized Low Density Lipoprotein Receptor-1 in Early Atherosclerosis Rats[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(6): 157-161.
DOI：

HU Yao-hong, YANG Qiao, GAO Li-juan. Influence of Maixuekang Capsule on Lipid Metabolism and Expression of Lectin Like Oxidized Low Density Lipoprotein Receptor-1 in Early Atherosclerosis Rats[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(6): 157-161. DOI： 10.11653/syfj2014060157.

摘要

目的：探讨脉血康胶囊对动脉粥样硬化（AS）大鼠脂质代谢及血凝素样氧化低密度脂蛋白受体1（LOX-1）表达的影响及其可能机制。方法：将32只健康雄性SD大鼠随机分正常对照组，模型组，脉血康高、低剂量组（1.5，0.3 g·kg-1）。采用高脂饮食+腹腔注射维生素D3的方法建立大鼠早期AS模型，治疗组在高脂饮食的同时每天给予相应药物灌胃，连续喂养10周。检测血脂、同型半胱氨酸（HCY）、过氧化氢酶（CAT），用ELISA法检测血清氧化低密度脂蛋白（ox-LDL）及主动脉壁血凝素样氧化低密度脂蛋白受体1（LOX-1）的表达，取大鼠胸主动脉作病理学观察。结果：与正常对照组相比，模型组血清TC，TG，LDL-C，HCY显著升高（P<0.05或P<0.01），HDL-C，CAT显著降低（P<0.01），ox-LDL和LOX-1表达（34.17±13.4），（1.58±0.08）μg·L-1较正常对照组（20.37±2.5），（1.44±0.08）μg·L-1明显升高（P<0.05或P<0.01）；脉血康胶囊可以明显降低TC，TG，LDL-C，HCY，升高HDL-C，CAT（P<0.05或P<0.01），下调ox-LDL（28.92±4.5，21.81±3.9）μg·L-1和LOX-1（1.45±0.07，1.39±0.09）μg·L-1的表达（P<0.05或P<0.01）。胸主动脉石蜡切片HE染色显示模型组内、中膜增厚，弹力纤维增生，平滑肌排列紊乱；与之相比脉血康各组内、中膜增厚不明显，弹力纤维轻度增粗，平滑肌细胞排列较整齐。结论：脉血康胶囊通过调节脂质代谢，减轻氧化损伤，降低血清ox-LDL水平及下调其特异性受体LOX-1的表达等环节，保护血管内皮，抑制动脉粥样硬化的发生发展。

Abstract

Objective: To investigate the influence and possible mechanism of Maixuekang capsule on lipid metabolism and expression of lectin like oxidized low density lipoprotein receptor-1(LOX-1) in early atherosclerosis rats. Method: Thirty-two healthy male SD rats were randomly divided into normal control group

model control group

high and low dose groups(1.5

0.3 g·kg-1·d-1) of Maixuekang capsule. Model control group and Maixuekang capsule groups were intraperitoneally injected by 6×105 unit vitamin D3

and corresponding medicines were ig administrated every day on the basis of high-fat diet for 10 successive weeks. Levels of blood lipid profile

homocysteine(HCY)

catalase(CAT) were observed. The expression of oxidized low density lipoprotein(ox-LDL) in serum and LOX-1 in aortic wall were detected by ELISA. The pathology of thoracic aortas were observed. Result: In model control

the levels of TC

LDL-C

HCY were increased than normal control (P<0.05 or P<0.01)

HDL-C and CAT were decreased(P<0.01). The expression of ox-LDL and LOX-1 was significantly increased (P<0.05 or P<0.01) in model control(34.17±13.4)

(1.58±0.08) μg·L-1than the normal control(20.37± 2.5)

(1.44±0.08)μg·L-1. Maixuekang capsule decreased the levels of TC

LDL-C

HCY and increased the levels of HDL-C and CAT (P<0.05 or P<0.01);the expression of ox-LDL(28.92±4.5)

(21.81±3.9) μg·L-1and LOX-1(1.45±0.07)

(1.39±0.09)μg·L-1 was attenuated in all the treatment groups (P<0.05 or P<0.01). In model control

intima-media thickness

elastic fiber proliferation and disordered arrangement of smooth muscle cells were found in thoracic aortas by paraffin section and Hematoxylin staining.By contrast

intima-media thickness were not obvious

elastic fiber was mild wided and arrangement of smooth muscle cells in Maixuekang capsule group. Conclusion: The Maixuekang capsule can protect vascular endothelial cell and inhibit atherosclerosis by modulating the lipid metabolism

reducing the oxidative damage

and down-regulating the expression of ox-LDL and LOX-1.

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