Effect of Bushen Huoxue Formulae on Knee Osteoarthritis in Rabbits

YU Juan; LI Sha-sha; YE Ying-yi; GE Hong-qing; CHEN Wen-zhi; DENG Xiao-qiang; YU Yu-feng

doi:10.11653/syfj2014060171

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Effect of Bushen Huoxue Formulae on Knee Osteoarthritis in Rabbits

更新时间：2024-01-04

- Effect of Bushen Huoxue Formulae on Knee Osteoarthritis in Rabbits
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 6, Pages: 171-174(2014)
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- DOI：10.11653/syfj2014060171
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- Published：2014
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YU Juan, LI Sha-sha, YE Ying-yi, et al. Effect of Bushen Huoxue Formulae on Knee Osteoarthritis in Rabbits[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(6): 171-174.
DOI：

YU Juan, LI Sha-sha, YE Ying-yi, et al. Effect of Bushen Huoxue Formulae on Knee Osteoarthritis in Rabbits[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(6): 171-174. DOI： 10.11653/syfj2014060171.

摘要

目的：通过观察补肾活血方对 Hulth法复制兔膝骨性关节炎模型中软骨组织结构的改变及基质金属蛋白酶-2（MMP-2）、白细胞介素-1β（IL-1β）、肿瘤坏死因子（TNF-α）浓度的影响，探讨其对关节炎的治疗作用及机制。方法：48只新西兰兔，6只为正常对照，42只用Hulth法复制骨关节炎模型，4周后在模型中随机处死6只动物取材光镜下观察，造模成功后将其余36只随机分为治疗组和模型组，治疗组以补肾活血方15 g·kg-1·d-1ig，模型组予同体积生理盐水。给药后的第4，6，8周分别在2组随机处死6只动物，取48只动物股骨髁软骨做石蜡切片行 HE染色、观察软骨细胞形态，用ELISA试剂盒检测血清中IL-1β，TNF-α及关节液中MMP-2。结果：与正常组比较，模型组IL-1在第4，6，8周显著升高（P<0.01），MMP-2，TNF-α在第4，6周显著升高（P<0.01）。与模型组相比，Mankin's分数在第8周治疗组显著降低（P<0.001）；IL-1β在第8周治疗组血清中显著降低（P<0.05）；MMP-2在第6周关节液中显著降低（P<0.01）；TNF-α在第6周血清中显著降低（P<0.05）。结论：补肾活血方可降低IL-1β含量，在关节炎早期可降低MMP-2和TNF-α，并可改善软骨组织形态从而保护关节软骨。

Abstract

Objective: To investigate pathological changes of the cartilage and concentrations of interleukin-1β (IL-1β)

matrix metalloproteinases (MMPs) and blood tumor necrosis factor (TNF-α) by Bushen Huoxue formulae in treating rabbit knee osteoarthritis model copied by Hulth surgery. Method: Forty-eight rabbits were randomly divided control group with 6 rabbits

and model group are forty-two rabbits. The osteoarthritis model was established by Hulth surgery

among them 6 animals were randomly killed after modeling 4 weeks for microscope observation

and the remaining 36 rabbits were randomly divided into treatment group and model group

treatment group was given 15 g·kg-1 Bushen Huoxue

the model group was given the same dose of saline. After 4

8 weeks

6 animals were killed in each groups respectively to collect the medial femoral condyle to observe chondrocytes morphology by HE staining

meanwhile

the concentrations of IL-1β

MMP-2

TNF-α were assayed. Result: Compared with the normal group

in model group

concentration of IL-1β was significantly higher at 4

8 weeks (P<0.01); the concentration of MMP-2 and TNF-α was significantly higher in the 4th and 6th weeks(P<0.01). Compared with the model group

the Mankin's score in treatment group was much significantly lower(P<0.001) in 8 week. the serum concentration of IL-1β significantly was lower (P<0.05) in 8 week

the concentration of MMP-2 in joint fluid was significantly lower (P<0.01) in 6 week

the serum concentrations of TNF-α was significantly lower (P<0.05) in 6 week. Conclusion: Bushen Huoxue treatment can reduce the concentrations of IL-1β

MMP-2 and TNF-α

it can change the form of cartilage tissue so as to protect the articular cartilage in arthritis.

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Related Institution

Faculty of Environment and Life，Beijing University of Technology

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

Shaanxi University of Chinese Medicine

Affiliated Hospital of Shaanxi University of Chinese Medicine

The Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine (TCM)

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