Objective: To optimize purification technology parameters of total flavonoids from flowers of Camellia semiserrata with HP-20 macroporous resin. Method: With elution ratio and purity of total flavonoids as indexes

technology parameters of HP-20 macroporous resin were investigated by single factor test

including static saturated adsorption amount

dynamic saturated adsorption amount

the concentration and amount of alcohol

et al. Result: Static saturated adsorption amount was 50.5 mg per gram resin.Optimum purification technology was as following:the volume of sample liquid 9 BV

washed impurity with 3 BV of deionized water and 3 BV of 15% ethanol

eluted with 10.5 BV of 35% ethanol at flow rate of 2 BV·h-1.Under these process conditions

purity of total flavonoids was up to 54.24%

elution rate of total flavonoids was 29.83%. Conclusion: HP-20 macroporous resin had good purification effects on total flavonoids from flowers of C. semiserrata

this optimized purification process was reasonable

stable and feasible.