Objective: To optimize preparation technology of total saponins from Panax notoginseng transdermal gel ointments and establish its content determination method. Method: With adhesive power

appearance

coating properties

et al as comprehensive indexes

molding technology of total saponins from P. notoginseng gel ointments was optimized by orthogonal test.The content of total saponins from P. notoginseng was determined by HPLC. Result: The best formulation was as following:NP-700 and CMC (4:1) 5 g

kaolin 3.5 g

aluminium glycinate 0.18 g

glycerin and propylene glycol (2:1) 25 g

PVP-K90 0.5 g

PVPP 0.5 g

PVA 0.25 g.The linear range of notoginsenoside R1

ginsenoside Rg1 and ginsenoside Rb1 were 0.42-3.36 μg(r=0.999 6)

4.82-38.56 μg(r=0.999 4)

1.24-9.92 μg(r=0.999 7). Conclusion: This optimized preparation technology of gel ointments was stable and practical.Established HPLC method was simple

reliable

and suitable for content determination and quality control of the preparation.