WANG Xiao-ping, WANG Jin, TANG Can-hui, et al. Optimization of Purification Technology of Tannin in[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(7): 24-27.
DOI:
WANG Xiao-ping, WANG Jin, TANG Can-hui, et al. Optimization of Purification Technology of Tannin in[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(7): 24-27. DOI: 10.11653/zgsyfjxzz2013070024.
Optimization of Purification Technology of Tannin in
Objective: To optimize purification technology conditions of tannin from Diascorea cirrhosa. Method: With the content and retention rate of tannin as indexes
different macroporous resin properties of absorption and desorption were observed by static absorption method
purification technology conditions of tannin from D. cirrhosa was optimized by single factor test and L9(34) orthgonal design. Result: AB-8 type macroporous resin was chosen
its optimum purification technology conditions were as followings:sample amount no more than 17.25 mg per gram of resin
washed impurity with 3 BV water
and eluted by 1.5 BV 40% ethanol with flow rate of 0.5 BV·h-1
collected the eluent.The content and retention rate of tannin were 82.02%
64.39%
respectively. Conclusion: This optimized technology was stable
feasible and reasonable
it could provide experimental basis for industrial purification of tannin in D. cirrhosa.
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