Optimization of Extraction Technology for Total Flavonoids from Xinjiang  Fruit by Response Surface Methodology

OUYANG Yan; LIU Wei; ZHAO Xiao-nan

doi:10.11653/zgsyfjxzz2013070046

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Optimization of Extraction Technology for Total Flavonoids from Xinjiang Fruit by Response Surface Methodology

更新时间：2024-01-04

- Optimization of Extraction Technology for Total Flavonoids from Xinjiang Fruit by Response Surface Methodology
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 7, Pages: 46-49(2013)
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- DOI：10.11653/zgsyfjxzz2013070046
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- Published：2013
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OUYANG Yan, LIU Wei, ZHAO Xiao-nan. Optimization of Extraction Technology for Total Flavonoids from Xinjiang Fruit by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(7): 46-49.
DOI：

OUYANG Yan, LIU Wei, ZHAO Xiao-nan. Optimization of Extraction Technology for Total Flavonoids from Xinjiang Fruit by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(7): 46-49. DOI： 10.11653/zgsyfjxzz2013070046.

摘要

目的: 优选黑果小檗鲜果中总黄酮的提取工艺。方法: 采用紫外分光光度法测定总黄酮含量

在单因素试验基础上

以乙醇体积分数、料液比、提取时间为自变量

进行三因素三水平Box-Behnken试验设计

利用响应面分析法优化新疆黑果小檗鲜果总黄酮提取条件。结果: 黑果小檗鲜果中总黄酮的最佳提取工艺为乙醇体积分数43%

提取温度80 ℃

料液比1:33

提取时间2 h;总黄酮提取量24.75 mg·g-1

达到预测值25.37 mg·g-1的97.6%。结论: 采用响应面法优化黑果小檗鲜果中总黄酮的提取工艺合理可行。

Abstract

Objective: To optimize extraction technology of total flavonoids from Xinjiang Berberis heteropoda fruit. Method: The content of total flavonoids was determined by UV

which was as dependent variable

based on single factor test

with solid-liquid ratio

extraction time and ethanol concentration as independent variables

three factors and three levels Box-Behnken design was adopted

extraction technology conditions of total flavonoids from Xinjiang B. heteropoda fruit were optimized by response surface methodology. Result: Optimal extraction conditions were as followings:extracted 2 h with 33 times the amount of 43% ethanol at 80 ℃

yield of total flavonoids was 24.75 mg·g-1

which accounted for 97.6% of the predicted value(25.37 mg·g-1). Conclusion: Response surface methodology to optimize extraction technology of total flavonoids from Xinjiang B. heteropoda fruit was feasible and reasonable.

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