Screening of Reference Substances for Content Determination of Total Saponins in

CHEN Xu; DANG Xiao-fang; CAO Sa-li; LIN Long-fei; NI Jian; FENG Li-jun

doi:10.11653/zgsyfjxzz2013070066

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Screening of Reference Substances for Content Determination of Total Saponins in

更新时间：2024-01-04

- Screening of Reference Substances for Content Determination of Total Saponins in
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 7, Pages: 66-68(2013)
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- DOI：10.11653/zgsyfjxzz2013070066
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- Published：2013
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CHEN Xu, DANG Xiao-fang, CAO Sa-li, et al. Screening of Reference Substances for Content Determination of Total Saponins in [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(7): 66-68.
DOI：

CHEN Xu, DANG Xiao-fang, CAO Sa-li, et al. Screening of Reference Substances for Content Determination of Total Saponins in [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(7): 66-68. DOI： 10.11653/zgsyfjxzz2013070066.

摘要

目的: 考察选用不同皂苷类对照品为指标对样品中三七总皂苷含量测定的影响。方法: 以人参皂苷Rg1、人参皂苷Rb1、三七皂苷R1、三七总皂苷为对照品

香草醛-高氯酸显色

采用紫外分光光度法测定三七中三七总皂苷的含量。结果: 以不同对照品为指标测定三七总皂苷含量时

结果存在明显差异。结论: 紫外分光光度法测定三七及其制剂中总皂苷含量时

可用三七总皂苷作对照品

为含有三七的制剂含量测定提供较为可靠的实验数据。

Abstract

Objective: To investigate the effects of different control samples on content determination of total saponins from Panax notoginseng. Method: With ginsenosides Rg1

ginsenoside Rb1

notoginsenoside R1 and P. notoginseng saponins as reference substance

taking HClO4-vanilin staining method

the content of total saponins in P. notoginseng was determined by ultraviolet spectrophotometry. Result: There were significant differences in determination of the content of total content from P. notoginseng among groups of different references. Conclusion: With UV spectrophotometry as content determination method for total saponins from P. notoginseng

we could choose P. notoginseng saponins as a reference substance

which could provide more reliable experimental data for determining the content of P. notoginseng and its preparations.

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Related Institution

School of Chinese Materia Medica,Beijing University of Traditional Chinese Medicine

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Guangzhou University of Chinese Medicine

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