Determination of Five Alkaloids from  of Different Parts and Different Growth Years

CHEN Jing; WANG Shu-mei; MENG Jiang; SUN Fei; WANG Bai-ling; LIU Bei; LIANG Sheng-wang

doi:10.11653/zgsyfjxzz2013070080

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Determination of Five Alkaloids from of Different Parts and Different Growth Years

更新时间：2024-01-04

- Determination of Five Alkaloids from of Different Parts and Different Growth Years
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 7, Pages: 80-84(2013)
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- DOI：10.11653/zgsyfjxzz2013070080
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- Published：2013
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CHEN Jing, WANG Shu-mei, MENG Jiang, et al. Determination of Five Alkaloids from of Different Parts and Different Growth Years[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(7): 80-84.
DOI：

CHEN Jing, WANG Shu-mei, MENG Jiang, et al. Determination of Five Alkaloids from of Different Parts and Different Growth Years[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(7): 80-84. DOI： 10.11653/zgsyfjxzz2013070080.

摘要

目的: 对不同年限苦参药材茎、叶、芦头、侧根、根5个部分总生物碱与5种单体生物碱进行含量测定。方法: 采用高效液相色谱法分析不同年限苦参药材不同部位中5种指标性生物碱的含量。结果: 5种指标性生物碱在不同生长年限苦参不同部位的分布有不同特点

不同部位槐果碱的含量分布叶>茎>侧根>主根>芦头

苦参碱的分布是叶>茎>芦头>侧根>主根

氧化槐果碱的分布是侧根>主根>芦头>茎>叶

槐定碱的分布是侧根>主根>叶>茎>芦头

氧化苦参碱的分布是侧根>主根>芦头>茎>叶。随着生长年限的增加

各指标性生物碱成分含量随之增加

第4年有效成分增加幅度较小

其中主根中5种指标性成分含量依次为一年生13.58 mg·g-1

两年生20.49 mg·g-1

三年生27.74 mg·g-1

四年生31.32 mg·g-1。结论: 上述结果为合理开发利用苦参以及苦参的生长年限提供了实验依据。

Abstract

Objective: To determine the five alkaloids of different parts in S. flavescens of different growth years. Method: The five alkaloids in S. flavescens were analyzed by HPLC. Result: Five alkaloids of different parts in S. flavescens of different growth years had their distribution law. The content of sophocarpine's distribution in different parts was leaf>stem>lateral root>taproot>reed head; the distribution of matrine was leaf>stem>reed head>lateral root>taproot; the distribution of oxysophocarpine was lateral root>taproot>reed head>stem>leaf; the distribution of sophoridine was lateral root>taproot>leaf>stem>reed head; the distribution of oxymatrine was lateral root>taproot>reed head>stem>leaf. With the increase of growth years

the five alkaloids increased; the fourth year was tend to be gentle. For example

the content of the taproot was: 13.58 mg·g-1 (first year)

20.49 mg·g-1 (second year)

27.74 mg·g-1(third year)

31.32 mg·g-1(forth year). Conclusion: This research can provide data for rational utilization and growth years determination of S. flavescens.

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