Anti-hypertensive Effects of Extracts from Leaves of  through Activating PI3K/Akt Pathway on Human Endothelial Cells

FU Jian-jiang; WANG Xi-ling; LV Hong; LIU Rong; YIN Xiao-ying; ZHENG Yang-bin; LUO Yong-ming

doi:10.11653/zgsyfjxzz2013070159

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Anti-hypertensive Effects of Extracts from Leaves of through Activating PI3K/Akt Pathway on Human Endothelial Cells

更新时间：2024-01-04

- Anti-hypertensive Effects of Extracts from Leaves of through Activating PI3K/Akt Pathway on Human Endothelial Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 7, Pages: 159-164(2013)
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- DOI：10.11653/zgsyfjxzz2013070159
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- Published：2013
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FU Jian-jiang, WANG Xi-ling, LV Hong, et al. Anti-hypertensive Effects of Extracts from Leaves of through Activating PI3K/Akt Pathway on Human Endothelial Cells[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(7): 159-164.
DOI：

FU Jian-jiang, WANG Xi-ling, LV Hong, et al. Anti-hypertensive Effects of Extracts from Leaves of through Activating PI3K/Akt Pathway on Human Endothelial Cells[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(7): 159-164. DOI： 10.11653/zgsyfjxzz2013070159.

摘要

目的: 研究罗布麻叶提取物(extracts from leaves of Apocynum venetum

ELA)的抗高血压作用及其激活磷脂酰肌醇3激酶(PI3K)和(或)蛋白激酶B(Akt)信号转导通路促进一氧化氮释放的作用。方法: 采用双肾双夹法建立Beagle犬高血压模型

随后按血压值将Beagle犬随机分为模型组(ig

生理盐水)、马来酸依那普利组(ig

1.66 mg·kg-1)、ELA低剂量组(ig 23 mg·kg-1)、高剂量组(ig ELA 46 mg·kg-1)。受试动物在单次给予上述药物后

每隔1 h记录1次动物血压

连续8 h

观察ELA的降压作用;采用细胞培养法

观察ELA对内皮细胞(EAhy 926)一氧化氮(NO)产量和内皮型一氧化氮合酶(eNOS)活性的影响;采用Western blot法

观察ELA对eNOS

磷酸化内皮型一氧化氮合酶(p-eNOS)、磷酸化磷脂酰肌醇3激酶(p-PI3K)以及磷酸化蛋白激酶B (p-Akt)表达量的影响。结果: 给药后

ELA高、低剂量组Beagle犬收缩压分别为(140.4±7.5)

(136.2±6.8) mmHg;舒张压分别为(63.5±3.3)

(72.2±4.4) mmHg;平均血压分别为(88.9±4.7)

(93.3±5.2)mmHg

与给药前相比均显著降低(P<0.01);ELA可显著增加EA Hy926细胞NO的释放量(P<0.01)

提高eNOS活性(P<0.01);Western blot结果表明

ELA可显著增加p-eNOS

p-PI3K以及p-Akt的表达量

与对照组相比

具有显著差异(P<0.01)。结论: ELA具有明显的降压作用。激活血管内皮细胞内PI3K/Akt信号转导途径

从而促进eNOS磷酸化

提高eNOS活性、增加NO可能是ELA降压作用的机制之一。

Abstract

Objective: To investigate the anti-hypertensive effects of extracts from leaves of Apocynum venetum L. (ELA) and its mechanisms. Method: Twelve Beagle dogs were used to establish hypertensive mode by two-kidney two-clip method; endothelial cells were cultured to determine effects of ELA on nitric oxide(NO) output and endothelial nitric oxide synthase(eNOS) activities; then Western blot method was involved in current study to detect effects of ELA on eNOS

phospho-endothelial NOS(p-eNOS)

phosph-phosphponositide-3 kinase(p-PI3K) and phosph-protein kinase B(p-Akt) expression. Result: Contractive and diastolic pressure of Beagle dogs showed degression in dose-dependent manner after ELA was administrated (P<0.01). Moreover

NO yield and eNOS activities of EA hy 926 cells were increased significantly when these cells incubated with ELA (P<0.01). It was also showed that ELA enhanced expression of eNOS

p-eNOS

p-PI3K and p-Akt in endothelial cells. Conclusion: ELA shows significant anti-hypertensive properties. The mechanisms may be associated with activation of PI3K/Akt pathway in endothelium.

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