Compound Prescription Tongluo Jiunao Promotes Synaptic Plasticity of Nerve Cells

CHEN Jin-yan; CHEN Wen-ju; LI Jiao; DING Hai-min; LIANG Mi; WANG Feng; HUA Qian

doi:10.11653/zgsyfjxzz2013070164

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Compound Prescription Tongluo Jiunao Promotes Synaptic Plasticity of Nerve Cells

更新时间：2024-01-04

- Compound Prescription Tongluo Jiunao Promotes Synaptic Plasticity of Nerve Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 7, Pages: 164-167(2013)
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- DOI：10.11653/zgsyfjxzz2013070164
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- Published：2013
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CHEN Jin-yan, CHEN Wen-ju, LI Jiao, et al. Compound Prescription Tongluo Jiunao Promotes Synaptic Plasticity of Nerve Cells[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(7): 164-167.
DOI：

CHEN Jin-yan, CHEN Wen-ju, LI Jiao, et al. Compound Prescription Tongluo Jiunao Promotes Synaptic Plasticity of Nerve Cells[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(7): 164-167. DOI： 10.11653/zgsyfjxzz2013070164.

摘要

目的: 检测中药复方通络救脑及其主要成分三七总皂苷和京尼平苷对神经突触可塑性有无促进作用。方法: 实验分为对照组和给药组

采取细胞活力检测法

即CCK-8检测法检测药物对稳转的过表达的瑞典型突变淀粉前体蛋白(swAPP)突变的人神经母细胞瘤(SY5Y)细胞的增殖曲线的影响;通过Western blotting检测药物对于神经细胞突触相关蛋白树突棘肌动蛋白结合蛋白(drebrin)和突触后密度蛋白-95(PSD-95)的表达的变化;用神经生长因子(NGF)诱导PC12细胞长出神经突起后

检测药物对于细胞突起的作用。结果: 复方通络救脑及其主要组成成分京尼平苷和三七总皂苷不影响过表达的瑞典型突变淀粉样前体蛋白的神经母细胞瘤(swAPP-SY5Y)的细胞增殖曲线

且复方通络救脑中京尼平苷能够显著增加swAPP-SY5Y细胞drebrin和PSD-95的表达(P<0.01)

显著增加PC12细胞总突起数(P<0.01);而三七总皂苷没有相应的作用。结论: 复方通络救脑能促进神经细胞的突触可塑性

初步断定起主要作用的成分是京尼平苷而不是三七总皂苷。

Abstract

Objective: To investigate whether the compound prescription Tongluo Jiannao and its main ingredients like geniposide (Gp) and Panax notoginseng Saponins (PNS) can promote the synaptic plasticity of nerve cells. Method: This experiment was divided into control group and treatment groups.Cell counting kit-8(CCK-8) kit was used to detect the cell proliferation curve after adding the drugs into the medium at the time interval 6

48 h. Western blotting was used to assay the synapse-associated proteins

such as drebrin and postynaptic density protein-95(PSD-95).PC12 cells induced by nerve growth factor (NGF) were used to observe if the drugs improve the amount of synapses. Result: Tongluo Jiannao

Gp and PNS couldn't influence the cell proliferation curve

and Gp could improve the expression of drebrin and PSD-95 significantly (P<0.01)

Gp also could increase the amount of synapses (P<0.01). Conclusion: Tongluo Jiannao and geniposide could promote synaptic plasticity of nerve cells.

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