Influence of  Polysaccharide with Interferon- inducible Protein 10 on the Differentiation of Helper T Cells in Bearing Mice

SHE Yan-ling; HE Yan-li; DU Biao-yan; XU Qian; LUO Hui; SU Ning; SU Jun-fang; LIU Juan

doi:10.11653/zgsyfjxzz2013070208

您当前的位置：

首页 >

文章列表页 >

Influence of Polysaccharide with Interferon- inducible Protein 10 on the Differentiation of Helper T Cells in Bearing Mice

更新时间：2024-01-04

- Influence of Polysaccharide with Interferon- inducible Protein 10 on the Differentiation of Helper T Cells in Bearing Mice
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 7, Pages: 208-211(2013)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.11653/zgsyfjxzz2013070208
  CLC：
- Published：2013
- 稿件说明：
移动端阅览
SHE Yan-ling, HE Yan-li, DU Biao-yan, et al. Influence of Polysaccharide with Interferon- inducible Protein 10 on the Differentiation of Helper T Cells in Bearing Mice[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(7): 208-211.
DOI：

SHE Yan-ling, HE Yan-li, DU Biao-yan, et al. Influence of Polysaccharide with Interferon- inducible Protein 10 on the Differentiation of Helper T Cells in Bearing Mice[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(7): 208-211. DOI： 10.11653/zgsyfjxzz2013070208.

摘要

目的: 探讨枸杞多糖(LBP)及枸杞多糖联合趋化因子(CXCL10)对实验性肝癌荷瘤小鼠辅助性T细胞分化的影响。方法: 建立H22肝癌小鼠皮下移植瘤模型

分为模型组、LBP高剂量组(ig 100 mg·kg-1)、LBP低剂量组(ig 50 mg·kg-1)、LBP+CXCL10组(ig 100 mg·kg-1+15 μg·kg-1)、CTX组(ip 20 mg·kg-1)

另设正常对照组。连续给药2周后小鼠眼球取血

颈椎脱臼处死。分离小鼠肿瘤、脾脏、胸腺

计算肿瘤抑制率、胸腺指数、脾指数

流式细胞术检测小鼠外周血T辅助细胞1/T辅助细胞2(Th1/Th2)分化情况。结果: 与模型组相比

LBP低、高剂量组、LBP+CXCL10组对肿瘤抑制率分别为37.83%

12.50%

14.14%; Th1/Th2分别4.44±3.05

2.48±2.93

4.36±1.96

其中LBP低剂量组及LBP+CXCL10组P<0.05。结论: 低剂量LBP及LBP+XCL10能显著提高H22肝癌荷瘤小鼠Th1/Th2比率。

Abstract

Objective: To investigate the influence of Lycium bararum polysaccharide (LBP) and LBP with interferon-inducible protein 10(CXCL10) on the differentiation of helper T cells in bearing mice. Method: H22 bearing mice model was established and randomized into six groups: the model group

high-and low dose group

LBP with CXCL10 group

CTX group

control group. Serum was separated from eye ball after two weeks of treatment. tumors

spleen

thymus were separated and calculated anti-tumor rate

thymus index and spleen index. Flow cytometry was used to detect the differentiation of Th1/Th2 in mice peripheral blood. Result: Compared with model group

tumor inhibitory rate in low-and high dose group

LBP with CXCL10 group was 37.83%

12.50%

14.14%; the rate of Th1/Th2 was 4.44±3.05

2.48±2.93

4.36±1.96

among them LBP low dose group and LBP with CXCL10 group was obvious(P<0.05). Conclusion: Low dose of LBP and LBP with CXCL10 can increase the rate of Th1/Th2 markedly in bearing mice.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Combined and Scutellariae Barbatae Herba Superfine Powder on Expressions of Bcl-2 and Bax Proteins in Tumor Tissues of Transplanted Hepatocarcinoma in Mice

Related Author

LIU Jin

Related Institution

No data

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰