CHEN Qiong, LI Dan, ZHOU Ji-lei. Optimization of Extraction Technology of Compound Lobelia Injection by Synergistic Action of Cellulase and Ultrasonic Method[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(7): 13-15.
DOI:
CHEN Qiong, LI Dan, ZHOU Ji-lei. Optimization of Extraction Technology of Compound Lobelia Injection by Synergistic Action of Cellulase and Ultrasonic Method[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(7): 13-15. DOI: 10.13422/j.cnki.syfix.2014070013.
Optimization of Extraction Technology of Compound Lobelia Injection by Synergistic Action of Cellulase and Ultrasonic Method
Objective: To optimize extraction technology of compound lobelia injection by synergistic action of cellulase and ultrasonic method. Method: Under these established ultrasonic conditions
orthogonal test was adopted to investigate effects of enzyme dosage
enzymolysis time
pH and temperature on yield of scutellarin in compound lobelia injection.The content of scutellarin was determined by HPLC. Result: Optimum technology conditions were as follows:enzymolysis temperature at 40 ℃
pH of 6.0
cellulase dosage 1 g(6.7 mg·g-1)
enzymolysis time 30 min;Under these conditions
the concentration of scutellarin in compound lobelia injection was 4.68 g·L-1
which was significantly more than 1.34 g·L-1 through decocting technology contained in registration standards
3.84 g·L-1 through single ultrasonic extraction and 2.26 g·L-1 through single cellulose extraction. Conclusion: Compared with the original extraction method contained in registration standards
synergistic action of cellulase and ultrasonic method had advantages of high extraction efficiency
less production cost and shorter production duration
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