SHI Xiao-wei, LIU Wen, CHEN Da-ye, et al. Optimization of Utrafiltration Purification Technology of Gegen Qinlian Extract[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(7): 19-22.
DOI:
SHI Xiao-wei, LIU Wen, CHEN Da-ye, et al. Optimization of Utrafiltration Purification Technology of Gegen Qinlian Extract[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(7): 19-22. DOI: 10.13422/j.cnki.syfix.2014070019.
Optimization of Utrafiltration Purification Technology of Gegen Qinlian Extract
Objective: To optimize ultrafiltration purification technology parameters of Gegen Qinlian extract. Method: With composite score of transfer rates of puerarin
baicalin
berberine hydrochloride and ammonium glycyrrhetate as index
orthogonal test was adopted to optimize purification technology of Gegen Qinlian extract by taking ultrafiltration pressure
liquid temperature and solvent amount as factors. Result: Hollow-fiber membrane with 5000 relative molecular weight had better separation efficiency as others
optimum ultrafiltration technology parameters were as follow:ultrafiltration pressure 0.025 MPa
liquid temperature 40 ℃
2 times the amount of solvent;Transfer rates of puerarin
baicalin
berberine hydrochloride and ammonium glycyrrhetate were 82.78%(RSD 2.76%)
88.63%(RSD 1.43%)
89.75%(RSD 2.52%)
59.91%(RSD 1.97%). Conclusion: Hollow-fiber membrane could be selectively used in purification of liquid
it could increase retention rate of active ingredient in Gegen Qinlian extract.
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