Optimization of Clarification Process for Water Extract of  Leaves by ZTC 1+1-Ⅱ Type Clarifying Agent

DONG Gang; LIU Hai-yan; HAN Liu; CHENG Yan-bin; LI Ping-ya; LU Dan

doi:10.13422/j.cnki.syfix.2014070023

您当前的位置：

首页 >

文章列表页 >

Optimization of Clarification Process for Water Extract of Leaves by ZTC 1+1-Ⅱ Type Clarifying Agent

更新时间：2024-01-04

- Optimization of Clarification Process for Water Extract of Leaves by ZTC 1+1-Ⅱ Type Clarifying Agent
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 7, Pages: 23-26(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfix.2014070023
  CLC：
- Published：2014
- 稿件说明：
移动端阅览
DONG Gang, LIU Hai-yan, HAN Liu, et al. Optimization of Clarification Process for Water Extract of Leaves by ZTC 1+1-Ⅱ Type Clarifying Agent[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(7): 23-26.
DOI：

DONG Gang, LIU Hai-yan, HAN Liu, et al. Optimization of Clarification Process for Water Extract of Leaves by ZTC 1+1-Ⅱ Type Clarifying Agent[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(7): 23-26. DOI： 10.13422/j.cnki.syfix.2014070023.

摘要

目的：优选ZTC 1+1-Ⅱ型澄清剂处理黄瑞香叶水提液的工艺条件。方法：以祖师麻甲素保留率和固形物去除率为指标，通过单因素试验考察药液质量浓度、温度、搅拌速度、水浴保温时间、澄清剂组份加入顺序和加入量对澄清效果的影响。采用HPLC测定祖师麻甲素含量，流动相甲醇-0.5%乙酸（25：75），检测波长327 nm。结果：最佳澄清工艺为药液生药质量浓度0.1 g·mL-1，温度80 ℃，加入B组份溶液后加入A组份溶液，加入量分别为6%，3%，搅拌速度100 r·min-1，水浴保温时间10 min；祖师麻甲素保留率74.29%，固形物去除率38.80%。结论：ZTC 1+1-Ⅱ型澄清剂处理黄瑞香叶提取液的澄清效果良好，优选的澄清工艺稳定可行。

Abstract

Objective: To optimize clarification process of water extract of Daphne giraldii leaves by ZTC 1+1-Ⅱ type clarifying agent. Method: With retention rate of daphnetin and solid removal rate as indexes

single factor tests were adopted to optimize clarification process by taking extract concentration

reaction temperature

stirring speed

bath soaking time

adding order and amount of clarifying agents as factors.HPLC was adopted to determine the content of daphnetin with detection wavelength at 327 nm and mobile phase of methanol-0.5% acetic acid(25:75). Result: Optimal clarification process was as follows:concentrated water extract to 0.1 g·mL-1

reaction temperature at 80 ℃

added 6% of B component

then added 3% of A component

stirring speed of 100 r·min-1

bath soaking time for 10 minutes;Under these conditions

retention rate of daphnetin and solid removal rate were 74.29% and 38.80%

respectively. Conclusion: Clarification effect of ZTC 1+1-Ⅱ type clarifying agent for water extract of D. giraldii leaves is effective

and this optimized process was stable and feasible.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Measurement of Daphnetin Contents in Cortex Daphnes and Different Processed Drugs by HPLC

Optimization of Purification Technology of Total Coumarins in

Optimization of Clarification Process of Shenyu Oral Liquid

Optimization of Softening Cutting Process of by Orthogonal Design

Optimization of Clarification Process of Gastrodiae Rhizoma Extract with Chitosan Flocculating Method

Related Author

ZHANG Xian

SUN Cheng-rong

ZHANG Su-heng

TANG Ying-shuang

KANG A-long

CHEN Liu-qin

ZHU Shuang-yan

ZHANG Li

Related Institution

No data

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰