Preparation of Hypericin by Polymer Nanospheres

WANG Jin-lin; LI Qian-qiong; WANG Xiao-li; ZHANG Jun-song

doi:10.13422/j.cnki.syfix.2014070034

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Preparation of Hypericin by Polymer Nanospheres

更新时间：2024-01-04

- Preparation of Hypericin by Polymer Nanospheres
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 7, Pages: 34-36(2014)
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- DOI：10.13422/j.cnki.syfix.2014070034
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- Published：2014
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WANG Jin-lin, LI Qian-qiong, WANG Xiao-li, et al. Preparation of Hypericin by Polymer Nanospheres[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(7): 34-36.
DOI：

WANG Jin-lin, LI Qian-qiong, WANG Xiao-li, et al. Preparation of Hypericin by Polymer Nanospheres[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(7): 34-36. DOI： 10.13422/j.cnki.syfix.2014070034.

摘要

目的：利用聚合物纳米微球材料分离纯化金丝桃素。方法：采用PEK微球对金丝桃素提取物进行富集，运用PDCX微球纯化，通过ODS柱制备高纯度金丝桃素，通过单因素试验优选纯化工艺。采用HPLC测定金丝桃素含量，流动相甲醇-0.006 mol·L-1 Na2HPO4（7：1，加H3PO4调pH 6.5），检测波长590 nm。结果：PEK微球对金丝桃素的静态饱和吸附量8.5 mg·g-1，解析率89.6%，经PEK微球动态柱富集的金丝桃素样品纯度3.2%，回收率89.9%；经PDCX微球纯化后，金丝桃素纯度33.0%，回收率80.1%；经ODS柱制备的金丝桃素纯度达91.6%，回收率86.7%。结论：该制备工艺高效、环保、经济，适用于金丝桃素等不稳定性成分的分离纯化。

Abstract

Objective: To prepare high purity of hypericin by polymer nanospheres. Method: Hypericin extract was enriched by PEK nanospheres

then PDCX nanospheres further purification

high-purity of hypericin was prepared by ODS column

single factor tests were adopted to optimize purification technology.HPLC was employed to determined the content of hypericin with mobile phase of methanol-0.006 mol·L-1 Na2HPO4(7:1

added H3PO4 adjusted pH to 6.5)and detection wavelength of 590 nm. Result: Static saturated adsorption capacity of PEK nanospheres for hypericin was 8.5 mg·g-1 with elution ratio of 89.6%

after extract was enriched by PEK nanospheres column

purity of hypericin was 3.2% with recovery rate of 89.9%;Purity of hypericin was 33.0% through PDCX nanospheres chromatography

recovery rate was 80.1%;Hypericin with a purity of 91.6% was obtained by ODS column chromatography

recovery rate was 86.7%. Conclusion: This preparation process was efficient and economical

which was suitable for separation and purification of hypericin and other unstable ingredients.

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