Objective: To explore chemical composition in yellow hairs of rhizome of Cibotii Rhizoma and its material basis for topical hemostatic effect. Method: Chemical composition in yellow hairs of rhizome of Cibotii Rhizoma was extracted by ultrasound

Soxhlet's extractor and reflux methanol extraction technologies

each extract was determined by UPLC/Q-TOF-MS for qualitative analysis

mobile phase of acetonitrile(A)-water(B) gradient elution(0-5 min

5%-55% A;5-7 min

55%-85% A;7-15 min

85%-100% A;15-20 min

100% A)

detection wavelength was 254 nm

scanning range of m/z from 100 to 1 000

desolvation gas temperature at 350 ℃

volume flow of desolvation gas was 9 L·min-1

spray pressure was 2.4×105 Pa

capillary voltage was 4 000 V. Result: A total of 50 compounds were checked out by these three methods

38 compounds out of them were recommended for molecular formula and name.Eight compounds of Number 32-39 should be a series of triacylglycerols homologues. Conclusion: Relative molecular weight was concentrated within 200-800

including vegetable oils(triacylglycerols)

organic acids

organic acid esters

alkaloids

flavonoids

polyphenols

glycosides and so on

triacylglycerols of which may be the main ingredient in yellow hairs of rhizome of Cibotii Rhizoma.