Objective: To establish a quantitative method of quantitative analysis of multi-component with a single-marker (QAMS) for the content determination of flavonoid glycosides in Ginkgo biloba extract. Method: According to the operating method in the Pharmacopoeia

quercetin was used as the internal standard

the response factors of kaempferol

isorhamnetin and quercetin were respectively measured under a fixed ratio and different ratios of orthogonal design to calculate the relative correction factor

the relative correction factors were established after investigating the related influencing factors. In the content determination of the samples

the relative correction factor were used to calculate the content of kaempferol and isorhamnetin. The content of flavonoid glycosides was determined by kaempferol

isorhamnetin and quercetin. The results of the QAMS method were verified by comparing with the external standard method. Result: The relative correction factors of kaempferol and isorhamnetin were 1.020 and 1.033 respectively;RSD was 1.23%

1.03% respectively

demonstrating good reproducibility. Two methods did not show significant difference in results. Conclusion: The relative correction factors and the QAMS methods for the content determination of flavonoid glycosides in Ginkgo biloba extract were accurate and reliable. This method is suitable for routine

multi-batch content determination of flavonoid glycosides in Ginkgo biloba extract

bringing the convenience to the actual content determination

and has good practical value.