Simultaneous Determination of Seven Flavonoids Constitutes in Kudiezi Injection by RP-HPLC-DAD

MA Si-meng; LIU Rui; REN Xiao-liang; QI Ai-di; LI Yu-bo

doi:10.13422/j.cnki.syfix.2014070063

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Simultaneous Determination of Seven Flavonoids Constitutes in Kudiezi Injection by RP-HPLC-DAD

更新时间：2024-01-04

- Simultaneous Determination of Seven Flavonoids Constitutes in Kudiezi Injection by RP-HPLC-DAD
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 7, Pages: 63-66(2014)
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- DOI：10.13422/j.cnki.syfix.2014070063
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- Published：2014
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MA Si-meng, LIU Rui, REN Xiao-liang, et al. Simultaneous Determination of Seven Flavonoids Constitutes in Kudiezi Injection by RP-HPLC-DAD[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(7): 63-66.
DOI：

MA Si-meng, LIU Rui, REN Xiao-liang, et al. Simultaneous Determination of Seven Flavonoids Constitutes in Kudiezi Injection by RP-HPLC-DAD[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(7): 63-66. DOI： 10.13422/j.cnki.syfix.2014070063.

摘要

目的：建立HPLC-DAD同时测定苦碟子注射液中的7种黄酮类成分[木犀草素-7-O-龙胆二糖苷(LGT)、木犀草素-7-O-β-D-吡喃葡萄糖苷(LGCOP)、木犀草素-7-O-β-D-吡喃葡萄糖醛酸苷(LGCRP)、芹菜素-7-O-β-D-吡喃葡萄糖苷(AGCOP)、芹菜素-7-O-β-D-吡喃葡萄糖醛酸苷(AGCRP)、木犀草素(LI)、芹菜素(AGI)]含量的方法。方法：采用Waters Symmetry C18色谱柱（3.9 mm×150 mm，4.6 μm），流动相0.05%甲酸-乙腈和0.05%甲酸-水，梯度洗脱。流速1 mL·min-1，柱温35 ℃，检测波长340 nm。结果：7种待测成分的分离度良好，线性关系良好，加样回收率为99.0%~101.5%，均符合含量测定要求。建立的方法能够同时测定LGT，LGCOP，LGCRP，AGCOP，AGCRP，LI，AGI的含量，并对8批苦碟子注射液进行含量测定，各黄酮含量在0.157 9~103.4 mg·L-1。结论：该法简便可行，结果可靠，且能同时测定苦碟子注射液中7中黄酮类成分，可作为本制剂多成分内控质量的测定方法。

Abstract

Objective: To develop an analysis method of simultaneously determining [luteolin-7-O-gentibioside (LGT)

luteolin-7-O-β-D-glucopyranoside (LGCOP)

luteolin-7-O-β-D-glucuronopyranoside (LGCRP)

apigenin-7-O-β-D-glucopyranoside (AGCOP)

apigenin-7-O-β-D-glucuronopyranoside (AGCRP)

luteolin (LI) and apigenin (AGI)] in Kudiezi injection by RP-HPLC-DAD. Method: 0.05% formic acid-acetonitrile and 0.05% formic acid-water as the mobile phase

chromatographic column was Waters Symmetry C18(3.9 mm×150 mm

4.6 μm) in gradient elution mode with velocity:1 mL·min-1

column temperature 35 ℃

detection wavelength of 340 nm. Result: There were perfect chromatogram with good separation. All the 7 calibration curves showed a good linear relationship. The recoveries were 99.0%-101.5%

according to the requirement. The developed method was successfully applied for the simultaneous determination of the 7 components (LGT

LGCOP

LGCRP

AGCOP

AGCRP

AGI). After the determination of 8 batch of Kudiezi injection

the content of 7 flavonoids was in 0.157 9-103.4 mg·L-1. Conclusion: The method is convenient

specific

and can be used for the flavonoids constitutes quality control of Kudiezi injection.

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