Determination Related Substances and Content in  of Ammonium Glycyrrhizinate by HPLC

ZHAO Bo; ZHANG Hui; CHEN Ying; QIAO Jia

doi:10.13422/j.cnki.syfix.2014070073

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Determination Related Substances and Content in of Ammonium Glycyrrhizinate by HPLC

更新时间：2024-01-04

- Determination Related Substances and Content in of Ammonium Glycyrrhizinate by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 7, Pages: 73-77(2014)
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- DOI：10.13422/j.cnki.syfix.2014070073
  CLC：
- Published：2014
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ZHAO Bo, ZHANG Hui, CHEN Ying, et al. Determination Related Substances and Content in of Ammonium Glycyrrhizinate by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(7): 73-77.
DOI：

ZHAO Bo, ZHANG Hui, CHEN Ying, et al. Determination Related Substances and Content in of Ammonium Glycyrrhizinate by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(7): 73-77. DOI： 10.13422/j.cnki.syfix.2014070073.

摘要

目的：建立测定甘草酸单铵盐原料药的有关物质及其在不同产地甘草中含量的方法。方法：采用HPLC，Ecosil-C18色谱柱（4.6 mm×250 mm，5 μm），以磷酸盐缓冲液（pH 7.0）-乙腈（80：20）为流动相，流速1.0 mL·min-1，柱温30 ℃，检测波长250 nm。结果：甘草酸单铵盐与有关物质及各降解成分完全分离，检测限为1.52 ng，定量限为4.23 ng；甘草酸单铵盐含量在0.025 6~0.409 9 g·L-1进样量与峰面积呈良好线性关系（r=0.999 9），精密度良好（RSD 0.60%），平均回收率99.51%（n=9）。结论：该方法简便、准确、专属性好、灵敏度高，可用于甘草酸单铵盐原料的有关物质检查及其在不同产地甘草中的含量测定。

Abstract

Objective: To establish a method to determine related substances and content in Glycyrrhizia uralensis of ammonium glycyrrhizinate. Method: HPLC was adopted for the determination. The separation was performed on Ecosil-C18 column(4.6 mm×250 mm

5 μm)

column temperature at 30 ℃ with mobile phase consisted of phosphate buffer solution(pH=7.0)-acetonitrile(80：20). The flow rate was set at 1.0 mL·min-1 with its detection at 250 nm. Result: Related substances and degraded substances were completely separated from ammonium glycyrrhizinate

the least detective range was 1.52 ng

the least quantification range of 4.23 ng. The content was a good linear relationship within the range of 0.025 6-0.409 9 g·L-1(r=0.999 9).The precision in a day is good (RSD 0.60%). The average recovery was 99.51%(n=9). Conclusion: The method is simple

accurate

specific and sensitiveness for the determination of ammonium glycyrrhizinate related substances and its content.

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