Objective: To develop an HPLC fingerprint for the different compatibility proportion in Radix Paeoniae Rubra and Rhei Radix et Rhizoma. Method: Agilent Eclipse XDB-C18 (4.6 mm×250 mm

5 μm) was adopted;the mobile phase was acetonitrile (A)-0.1% phosphoric acid solution (B) with gradient elution at a flow rate of 1.0 mL·min-1

and the detection wavelength was set at 267 nm (0-13 min)

258 nm (13-20 min)

230 nm (20-45 min)

254 nm (45-100 min). Result: An HPLC fingerprint for the different compatibility proportion in Radix Paeoniae Rubra and Rhei Radix et Rhizoma was developed. A total of 18 chromatographic peaks and 9 chromatographic peaks were identified. Through studying 5 proportion of fingerprint of Radix Paeoniae Rubra and Rhei Radix et Rhizoma

the main components of two herbs belonging were preliminary identified. Conclusion: This method is dependable

simple and practical

and it may be used to identify component and control quality of Radix Paeoniae Rubra and Rhei Radix et Rhizoma.