Analysis on Dehydroeffusol Content of Cortex and Pith of  in Different Growing Stage

GUO Zhen-yu; LI Gui-yun; SUN Xue; DUAN Tian-xuan; HUANG Jian-mei

doi:10.13422/j.cnki.syfix.2014070104

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Analysis on Dehydroeffusol Content of Cortex and Pith of in Different Growing Stage

更新时间：2024-01-04

- Analysis on Dehydroeffusol Content of Cortex and Pith of in Different Growing Stage
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 7, Pages: 104-106(2014)
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- DOI：10.13422/j.cnki.syfix.2014070104
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- Published：2014
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GUO Zhen-yu, LI Gui-yun, SUN Xue, et al. Analysis on Dehydroeffusol Content of Cortex and Pith of in Different Growing Stage[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(7): 104-106.
DOI：

GUO Zhen-yu, LI Gui-yun, SUN Xue, et al. Analysis on Dehydroeffusol Content of Cortex and Pith of in Different Growing Stage[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(7): 104-106. DOI： 10.13422/j.cnki.syfix.2014070104.

摘要

目的：测定和比较两个产地不同生长时间灯心草皮部和髓部中去氢厄弗酚（DHE）的含量。方法：采用高效液相色谱（HPLC），Agilent Eclipse Plus-C18 色谱柱（4.6 mm×250 mm，5 μm），流动相甲醇-水（57：43），流速1.0 mL·min-1，检测波长270 nm，柱温30 ℃。结果：灯心草髓部中DHE的含量均明显高于同期皮部；两产地灯心草髓部中去氢厄弗酚的含量，在生长后期差异增大；两产地各时期灯心草皮部中去氢厄弗酚的含量较低，但在灯心草全草中，皮部所含DHE量与髓部相当。结论：H省和J省灯心草中DHE含量差异较大。

Abstract

Objective: To determine and compare the dehydroeffusol(DHE) content in rush pith and cortex of Juncus effusus collected in 2 places and different time. Method: The content of dehydroeffusol was determined by HPLC.An Agilent Eclipse Plus-C18 column (4.6 mm×250 mm

5 μm) was employed

the mobile phase of MeOH-H2O(57:43) was used at the flow rate 1.0 mL·min-1 and the signal was detected at 270 nm. Result: The content of DHE in pith was significantly higher than that in cortex in the same period;the difference of DHE contents given by the 2 producing areas increased with time;despite the cortex of J. effusus was contained little DHE per gram

it roughly contributed as much as DHE which contributed by the pith in the whole plant. Conclusion: The quality of J. effusus in 2 places have large differences.

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