XU Liang, LIU Chun-sheng, YANG Yan-yun, et al. Study on Identification of an Unknown Sample with DNA Barcoding[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(7): 127-129.
DOI:
XU Liang, LIU Chun-sheng, YANG Yan-yun, et al. Study on Identification of an Unknown Sample with DNA Barcoding[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(7): 127-129. DOI: 10.13422/j.cnki.syfix.2014070127.
Study on Identification of an Unknown Sample with DNA Barcoding
Objective: To identify an unknown broken plant sample. Method: In this work
the method included macroscopical identification
microscopic identification
DNA barcoding identification was used. Using the DNA barcoding identification technology
DNA extraction
PCR amplification and sequencing
ITS sequence data were analyzed by three methods about the Blast comparison method
specific point method
system development method. Result: The unknown sample is the seeds of family umbrelliferae plant Foeniculum vulgare Mill. Conclusion: Traditional Chinese medicine authentication technology can solve the species identification problem of unknown sample in the actual work. DNA barcoding technology provide a new method for TCM authentication and play an important role of the identification of unknown sample.