Objective: To discuss the influence of serum containing Yiqi Tongluo Jiedu recipe and gefitinib on proliferation and apoptosis of lung cancer H1975 cells in vitro. Method: The medicated serum was prepared by the serum pharmacology method and divided into the low

medium

and high dose Yiqi Tongluo Jiedu recipe groups(15.75

31.5

63 g·kg-1)

the gefitinib group(22.5 mg·kg-1)

the gefitinib(22.5 mg·kg-1) combined with low

medium

and high dose Yiqi Tongluo Jiedu recipe groups

and 0.9%NaCl solution group. 6×104/mL the single cell suspension was planted into 96-well microplates

100 μL/well

1×105/mL the single cell suspension was added to 6-well microplates and cover glasses in 6-well microplates

2 mL/well. RMPI-1640 culture solution containing 10% the medicated serum was added to each well for 24

48

72 h behind cell adherent.The proliferation of H1975 cells was measured by CCK-8 method.The cell apoptosis was measured by flow cytometry(FCM) and Annexin V-FITC/PI. Immunohistochemistry was used to measure the expression levels of Caspase-3. Result: The inhibition effects of different dose of the medicated serum on lung cancer H1975 cells was found. The inhibiting rate was 23.81% and 42.86% in high dose Yiqi Tongluo Jiedu recipe and high dose Yiqi Tongluo Jiedu recipe combined with gefitinib at 72 h. The inhibitory effect was increased with the increased drug doses.Compared with NS group

each group containing drug serum could induce apoptosis of H1975 cells. The apoptosis rate was 10.29%

25.77% in high dose Yiqi Tongluo Jiedu recipe and high dose Yiqi Tongluo Jiedu recipe combined with gefitinib at 72 h. The apoptosis protein Caspase-3 expression was positively correlated with drug dose and action time

the high dose Yiqi Tongluo Jiedu recipe combined with gefitinib was the strongest. Conclusion: Yiqi Tongluo Jiedu recipe can inhibit proliferation and induce apoptosis of H1975 cells.The promotion of Caspase-3 protein expression may be one of the mechanisms of inducing apoptosis of H1975 cells.