Objective: To optimize purification process of gossypol from Gossypium herbaceum by macroporous adsorption resin. Method: Static and dynamic adsorption/desorption tests for gossypol were carried out to select proper macroporous resin

single factor test was adopted to select elution rate

other purification parameters were optimized by orthogonal test

such as pH of sample solution

ethanol concentration and so on. Result: H-103 resin was selected as the best one among 13 different resins

optimal process was determinded as follows:the concentration of gossypol in loading solution 90 mg·L-1 with pH of 7

the concentration of ethnol in loading solution 40% and in eluting solution 80%;Purity of gossypol was enhanced from 6.2% in extract to 83.6% in eluant with transfer rate of 76.7%.After evaporation and recycle of solvent

then gossypol was obtained by recrystallization in a mixture solvent of acetone-acitic acid. Conclusion: H-103 resin was suitable for purification of gossypol from G. herbaceum.