YANG Jing, PENG Teng, LING Zhi-qun, et al. Optimization of Purification Process for Total Organic Acids from Roots of [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(8): 25-27.
DOI:
YANG Jing, PENG Teng, LING Zhi-qun, et al. Optimization of Purification Process for Total Organic Acids from Roots of [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(8): 25-27. DOI: 10.13422/j.cnki.syfix.2014080025.
Optimization of Purification Process for Total Organic Acids from Roots of
Objective: To optimize purification process of total organic acids from roots of Aralia cordata. Method: The content of total organic acids was determined by acid-base titration.With purity and transferring rate of total organic acids as indexes
combining with organic solvent extraction and acid-base method
effects of extraction solvents
reagent for alkalization and its concentration
alkalinizing times and pH of acid settling on purification process of total organic acids were investigated by single factor tests. Result: Optimum purification technology was selected as follows:extracted four times with two-fold volume of petroleum ether
1.0% KOH as alkalization reagent
three times for alkalization
pH 1 of acid settling by hydrochloric acid
extracted three times with petroleum ether after acid settling;Under these purification conditions
purity of total organic acids increased from 14.71% in extract to 65.08% with transferring rate of 76.17%. Conclusion: This purification process was stable and feasible
which could provide experimental evidence for comprehensive utilization of A. cordata resources.
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