ZOU Da-jiang, HUANG Xian-ju, GAO Rui-xi, et al. HPLC Determination of Three Alkaloids in [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(8): 41-43.
DOI:
ZOU Da-jiang, HUANG Xian-ju, GAO Rui-xi, et al. HPLC Determination of Three Alkaloids in [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(8): 41-43. DOI: 10.13422/j.cnki.syfix.2014080041.
Objective: To establish a method for the determination of aconitine
mesaconitine and bullatine A in Aconitine brachypdum. Method: A C18 column(4.6 mm×250 mm
5 μm) was used at 30 ℃ with methanol-0.01% triethylamine (73:27) as the mobile phase at the flow rate of 1.0 mL·min-1 and UV detection wavelength was set at 240 nm. Result: The average recovery of three alkaloids was 98.88%(RSD 0.86%)
98.68%(RSD 0.48%)
and 98.94%(RSD 0.65%) respectively. Conclusion: The method is simple
accurate and reproducible
which can be used in the content determination of three alkaloids in A. brachypdum and provide a more reasonable reference to control the quality of A. brachypdum.