Content Determination of Ginsenoside Rg, Ginsenoside Re and Ginsenoside Rb in Shenmai Injection by UPLC

LIN Su-na; YU Chu-qin; LIN Hua-qing; GUO Yu-hai; LIN Di-shi; ZHENG Wen-zhong; HUANG Xiao-min

doi:10.13422/j.cnki.syfix.2014080048

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Content Determination of Ginsenoside Rg, Ginsenoside Re and Ginsenoside Rb in Shenmai Injection by UPLC

更新时间：2024-01-04

- Content Determination of Ginsenoside Rg, Ginsenoside Re and Ginsenoside Rb in Shenmai Injection by UPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 8, Pages: 48-50(2014)
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- DOI：10.13422/j.cnki.syfix.2014080048
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- Published：2014
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LIN Su-na, YU Chu-qin, LIN Hua-qing, et al. Content Determination of Ginsenoside Rg, Ginsenoside Re and Ginsenoside Rb in Shenmai Injection by UPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(8): 48-50.
DOI：

LIN Su-na, YU Chu-qin, LIN Hua-qing, et al. Content Determination of Ginsenoside Rg, Ginsenoside Re and Ginsenoside Rb in Shenmai Injection by UPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(8): 48-50. DOI： 10.13422/j.cnki.syfix.2014080048.

摘要

目的：采用超高效液相色谱法分离测定参麦注射液中人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1的含量。方法：采用ACQUITY UPLC HSS T3 （2.1 mm×50 mm，1.8 μm）色谱柱，流动相乙腈（A）-0.05%磷酸（B），梯度洗脱（0~5 min，19%A；5~12 min，19%~28%A；12~18 min，28%~40%A），流速0.3 mL·min-1，检测波长203 nm，柱温25 ℃。结果：人参皂苷Rg1、人参皂苷Re和人参皂苷Rb1分别在0.020 96~0.209 6，0.017 64~0.176 4，0.023 88~0.238 8 g·L-1与相应峰面积呈良好线性关系，平均加样回收率（n=6）分别为100.12%，100.08%，99.51%，RSD分别为1.60%，2.03%，1.15%。结论：与HPLC相比，UPLC在不影响分离效果的情况下可显著提高参麦注射液中人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1的分析速度，改善分析效果，同时可减少溶剂的消耗。该方法简便、快捷、准确、重复性好，可代替HPLC法用于参麦注射液的多指标质量控制。

Abstract

Objective: To establish a method to determine the content of ginsenoside Rg1

ginsenoside Re and ginsenoside Rb1 in Shenmai injection by ultra performance liquid chromatography(UPLC). Method: ACQUITY® UPLC HSS T3 column (2.1 mm×50 mm

1.8 μm)was used with mobile phase consisted of acetonitrile (A)-0.05% phosphoric acid solution (B) by gradient elution (0-5 min

19%A;5-12 min

19%-28%A;12-18 min

28%-40%A) at a flow rate of 0.3 mL·min-1;the wavelength of detector was 203 nm. Result: Ginsenoside Rg1

ginsenoside Re and ginsenoside Rb1had good linearity within the range of 0.020 96-0.209 6

0.017 64-0.176 4

0.023 88-0.238 8 g·L-1 respectively. The average recoveries(n=6)were 100.12%

100.08%

99.51% and RSDs were 1.60%

2.03%

1.15% respectively. Conclusion: UPLC method may greatly improve the separation efficiency and analysis speed in the case of ginsenoside Rg1

ginsenoside Re and ginsenoside Rb1 in Shenmai injection while reducing the solvent consumption. As an alternative of conventional HPLC

UPLC is more convenient

rapid

accurate and excellent repeatability.

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