Objective: Fingerprint analysis of chemical constituents of Marsdenia Tenacissima. Method: With the use of HPLC

TC-C18 column(4.6 mm×150 mm

5 μm)

acetonitrile-water was used as the mobile phase with gradient elution

detection wavelength at 210 nm

flow rate of 1.0 mL·min-1

temperature of column at 25 ℃. Result: The fingerprint of M. tenacissima was established using ZP-7tenacigenin B [11α-O-(2-methylbutyryl)-12β-O-acetyltenacigenin B] as reference substance. 17 common peaks were identified. The precision and repeatability of the relative retention time RSD were less than 3%

each producing area in the chromatogram of common peak area was more than 90%. 10 batches of similarity were between 0.75-0.98

there were some differences. Conclusion: The method is accurate and reproducible

which can provide the basis for quality evaluation and quality control of M. tenacissima

also lay the foundation for further development of M. tenacissima.