Study on Qualtaive Method of Salidroside of Artifical Cultivation Ultivation Rhodiola Rosea by HPLC-MS

Tsering Balme; ZHAO Xiao-ling; MA Xin-bin; WANG Jing; SHE Yong-xin; SE Zhu; WUJIN Cuomu; Selang Wazhen; Laba Cdan; Bama Yuzhen; LI Bao-hai

doi:10.13422/j.cnki.syfix.2014080079

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Study on Qualtaive Method of Salidroside of Artifical Cultivation Ultivation Rhodiola Rosea by HPLC-MS

更新时间：2024-01-04

- Study on Qualtaive Method of Salidroside of Artifical Cultivation Ultivation Rhodiola Rosea by HPLC-MS
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 8, Pages: 79-81(2014)
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- DOI：10.13422/j.cnki.syfix.2014080079
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- Published：2014
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Tsering Balme, ZHAO Xiao-ling, MA Xin-bin, et al. Study on Qualtaive Method of Salidroside of Artifical Cultivation Ultivation Rhodiola Rosea by HPLC-MS[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(8): 79-81.
DOI：

Tsering Balme, ZHAO Xiao-ling, MA Xin-bin, et al. Study on Qualtaive Method of Salidroside of Artifical Cultivation Ultivation Rhodiola Rosea by HPLC-MS[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(8): 79-81. DOI： 10.13422/j.cnki.syfix.2014080079.

摘要

目的：测定人工种植红景天不同药用部位花瓣、茎、根的含量。方法：取各1 g样品经过60%的乙醇溶解，超声波清洗器清洗50 min，过滤，旋蒸，加2 mL的甲醇，用微孔滤膜（0.45 μm）过滤，采用CAPCELL PAK C18柱（2.0 mm× 150 mm，i.d.，3 μm），柱温 30 ℃，波长278 nm；样品温度10 ℃，进样体积10 μL。流动相为甲醇-1%乙酸溶液 45：55，流速为1 mL·min-1进行定量分析。结果：有效部位中的红景天苷含量从高到低依次为花瓣（35.00mg）、根（18.89mg）、茎（1.59 mg）。结论：建立了红景天中红景天苷含量的高效液相色谱方法，可适用于不同药材中红景天苷含量的测定。

Abstract

Objective: To determine the petals

stems

roots planted content Rhodiola different medicinal parts。Method: Each 1 g sample is dissolved after 60% ethanol

ultrasonic cleaner cleaning 50 min

filtered

rotary evaporated

add 2 mL of methanol

microporous filter membrane (0.45 μm) filter using CAPCELL PAK C18 column ((2.0 mm× 150 mm

i.d.

3 μm )

column temperature 30 ℃

wavelength 278 nm

sample temperature 10 ℃

injection volume of 10 μL. The mobile phase was methanol-1% acetic acid solution 45:55

flow rate of 1 mL·min-1 for quantitative analysis. Result: The effective parts of salidroside content in the order of the petals (35.00 mg)

root (18.89 mg)

stems (1.59 mg). Conclusion: A detection of salidroside content in Rhodiola rosea was established by high performance liquid chromatography. It can be applied to the analysis on the salidroside in the different medicinal materials.

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