LI Tong-hui, LI Ye, CHEN Gang, et al. Study on the Quality Control for Self-microemulsifying Granule of Brucea Javanica Oil[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(8): 89-92.
DOI:
LI Tong-hui, LI Ye, CHEN Gang, et al. Study on the Quality Control for Self-microemulsifying Granule of Brucea Javanica Oil[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(8): 89-92. DOI: 10.13422/j.cnki.syfix.2014080089.
Study on the Quality Control for Self-microemulsifying Granule of Brucea Javanica Oil
Objective: To establish the methods of quality control for self-microemulsifying granule of Brucea Javanica Oil. Method: According to Chinese Pharmacopoeia
the quality of preparation was estimated. The HPLC method was used to determine the content of oleic acid and linoleic acid. The product was analyzed by HPLC
using Venusil XBP-NH2column (4.6 mm×250 mm
5 μm)
with methanol-water (95:5) as the mobile phase
the flow rate of 1.0 mL·min-1 and the detection wavelength of 242 nm. Result: The quality of preparation accorded with Chinese Pharmacopoeia regulation. The calibration curve showed good linearity over the range of 0.004 362-0.043 62 g·L-1 for oleic acid;the average recovery was 98.04%. The calibration curve showed good linearity over the range of 0.000 912-0.009 14 g·L-1 for linoleic acid;the average recovery was 101.60%. The content method is convenient
accurate and reduplicative. Conclusion: TLC and HPLC methods are simple
accurate and fast
which could be used effectively for the quality control of self-microemulsifying granule of Brucea Javanica Oil.
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QIN Jian-ping
LU Yan-qin
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LI Jia-chun
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