Effect of Ampelopsin on HSC-T6 Cell Proliferation and Content of Collage and Cytokine

ZOU Jing; WANG Xiao-qin; ZHENG Zuo-wen

doi:10.13422/j.cnki.syfix.2014080163

您当前的位置：

首页 >

文章列表页 >

Effect of Ampelopsin on HSC-T6 Cell Proliferation and Content of Collage and Cytokine

更新时间：2024-01-04

- Effect of Ampelopsin on HSC-T6 Cell Proliferation and Content of Collage and Cytokine
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 8, Pages: 163-165(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfix.2014080163
  CLC：
- Published：2014
- 稿件说明：
移动端阅览
ZOU Jing, WANG Xiao-qin, ZHENG Zuo-wen. Effect of Ampelopsin on HSC-T6 Cell Proliferation and Content of Collage and Cytokine[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(8): 163-165.
DOI：

ZOU Jing, WANG Xiao-qin, ZHENG Zuo-wen. Effect of Ampelopsin on HSC-T6 Cell Proliferation and Content of Collage and Cytokine[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(8): 163-165. DOI： 10.13422/j.cnki.syfix.2014080163.

摘要

目的：研究蛇葡萄素对大鼠肝星状细胞（HSC-T6）的增殖及胶原蛋白、细胞因子生成的影响。方法：对HSC-T6增殖的影响：用含10%胎牛血清的高糖DMEM培养液制备成HSC-T6细胞3×104个/mL细胞悬液100 μL/孔接种于96孔培养板。设细胞对照组、秋水仙碱1.0 mg·L-1组和100.0，75.0，50.0，25.0，12.5 mg·L-15个不同质量浓度蛇葡萄素组，置37 ℃ 5%CO2培养48 h。采用MTT法检测蛇葡萄素对大鼠肝细胞HSC-T6增殖的影响。对HSC-T6生成Ⅰ，Ⅲ，Ⅳ型胶原蛋白及细胞因子（TGF-β1，PDGF）含量的影响：取终质量浓度分别为50.0，25.0，12.5 mg·L-1蛇葡萄素，3×104个/mL细胞悬液100 μL/孔细胞液接种于96孔培养板。阳性组秋水仙碱的质量浓度为1.0 mg·L-1。置37 ℃ 5%CO2培养48 h。ELISA法检测HSC-T6细胞生成的胶原蛋白（Ⅰ，Ⅲ，Ⅳ）、转化生长因子（TGF-β1）及血小板衍生因子（PDGF）含量。结果：蛇葡萄素100.0，75.0 mg·L-1对HSC-T6细胞的增殖有明显抑制作用，其无毒浓度为50.0 mg·L-1。50 mg·L-1的蛇葡萄素可使HSC-T6细胞生成的Ⅰ，Ⅲ，Ⅳ型胶原蛋白，TGF-β1，PDGF含量明显低于细胞对照组（P<0.05），25 mg·L-1的蛇葡萄素也可使HSC-T6细胞生成的Ⅲ型胶原蛋白含量明显低于细胞对照组（P<0.05）。结论：蛇葡萄素能抑制HSC-T6增殖并可使HSC-T6生成的Ⅰ，Ⅲ，Ⅳ型胶原蛋白，TGF-β1，PDGF含量降低。

Abstract

Objective: To study the effect of ampelopsin on the HSC-T6 cell proliferation rate and the content of collage and cytokine Method: Effect of ampelopsin on the cell (HSC-T6) proliferation rate. High glucose DMEM medium containing 10% fetal bovine serum was used to prepare HSC-T6 cells 3×104 cells/mL suspension 100 μL/well

which was seeded in 96-well culture plates. The control group

colchicine 1.0 mg·L-1 group and 100.0

75.0

50.0

25.0

12.5 mg·L-1

5 different concentrations of ampelopsin groups were designed. Cells were cultured at 37 ℃ 5%CO2 for 48 h. The cell proliferation inhibition rate was measured by MTT。Effect of ampelopsin on the content of collage(Ⅰ

Ⅲ

Ⅳ) and cytokine (TGF-β1

PDGF) was dected by ELISA. Result: Ampelopsin at 100.0

75.0 mg·L-1 might effectively inhibit proliferation of HSC-T6 cells

and the non-toxic drug concentration was 50.0 mg·L-1

the contents of collage Ⅰ

Ⅲ

Ⅳ and TGF-β1

PDGF in HSC-T6 cells were significantly lower than control group at 50.0 mg·L-1 of ampelopsin (P<0.05)

the contents of collage Ⅲ in HSC-T6 cells was significantly lower than control group by ampelopsin at 25.0 mg·L-1(P<0.05). Conclusion: Ampelopsin could inhibit the proliferation of HSC-T6 and reduce the content of collage Ⅰ

Ⅲ

Ⅳ and TGF-β1

PDGF.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Optimization of Extraction Technology for by Microwave-assisted Semi-bionic Method

Study of Ampelopsin on Apoptosis in Human Gastric Cancer Cells

Effect of Carapax Trionycis in Inhibiting Activation of Rat Hepatic Stellate Cell Induced by TGF-

Effects of Polypeptides from Cervi Cornu Pantotrichum-collagen/chitosan Composite Materials Prepared by Different Crosslinking Methods on Osteoblasts Proliferation

Optimization of Extraction Technology for Ampelopsis grossedentata by Microwave-assisted Semi-bionic Method

Related Author

HUANG Ren-jie

NI Feng

TAO Lin

LV Lin-yan

GAO Jian-rong

YOU Peng-tao

LIU Yan-wen

HU Zu-liang

Related Institution

Fujian Health College

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰