Objective: To investigate the effect of LBJZ oils on tumor growth and pseudopodium formation of human lung cancer cell line A549 cells. Method: Cell tumorigenicity was assayed by soft agar colony formation after treating A549 cells with LBJZ oils of 25

50

100 mg·L-1 for 20 d. The cell cycle was analyzed by flow cytometry after treating A549 cells with LBJZ oils of 25

50

100 mg·L-1 for 24 h. The changing of cytoskeletal was detected by immunofluorescence dyeing after treating A549 cells with LBJZ oils of 100 mg·L-1 for 24 h. Result: LBJZ oils inhibited proliferation of A549 cells in a dose-dependent manner. There were significant differences between results of colony formation in control group and in all three dosage groups (P<0.01). LBJZ oils blocked cell cycle progression at G2 phase. Ratios of cells in G2 phase of medium and high dosage groups were 21.92% and 29.94% respectively

which showed significant differences compared with that of control group (P<0.05). LBJZ oils caused damage to the cytoskeleton of A549 cells

and promoted generation of pseudopodia. Conclusion: It suggested that LBJZ oils significantly inhibited proliferation of A549 cells

which may related to arrest of cell cycle and changes of cytoskeleton on A549 cells. However

it can also lead to cell migration and cancer.