Objective: To study sensitization potential of five kinds of traditional Chinese medicine injections (TCMI) with clearing-heat function by using the BALB/c popliteal lymph node assay. Method: Seventy female BALB/c mice were randomly divided into positive group (mercuric chloride)

negative control group (normal saline) and the traditional Chinese medicine injection (TCMI) groups

mice were injected subcutaneously (50 μL) with drugs into the right pad of hind foot. Dosages of each animal from normal saline group and mercuric chloride group were 50 μL 0.9%NaCl and 50 μg HgCl2

respectively. The traditional Chinese medicine (TCM) injection groups

were given 1.92 mL·kg-1 Zhongjiefeng injection(ZJF)

0.96 mL·kg-1 Qingkailing injection(QKL)

0.96 mL·kg-1 Chuanxinlian injection (CXL)

0.96 mL·kg-1 Chaihu(CH) and 0.48 mL·kg-1 Banlangen(BLG). The animals were killed on the 6th day

the popliteal lymph nodes(PLN) were isolated and weighed respectively to calculate the PLN weight index(WI).Then the PLN from four of ten mice in each group were fixed with paraformaldehyde solution for histopathologic examination;the other PLN were calculated cell index(CI). Result: Compared to untreated sides

WI and CI of the PLN from mercuric chloride group (WI=3.82±0.93

CI=7.40±0.63)

Zhongjiefeng injection(ZJF

WI=2.21±0.15

CI=5.23±0.18)

Qingkailing injection(QKL

WI=2.13±0.10

CI=5.17±0.18) and Chuanxinlian injection (CXL

WI=2.06±0.14

CI=5.04±0.27) reached to WI>2 and CI>5(P<0.05 or P<0.01)and Compared to normal saline groups(WI=1.10±0.14

CI=1.23±0.11)

their PLN were significantly enlarged

cells were increased.And their pathological examination showed that treated side PLN were enlarged with remarkable germinal center and increased high endothelial venules proliferation.And the responses in Chaihu injection (CH

WI=1.39±0.16

CI=3.15±0.21) or Banlangen injection (BLG

WI=1.38±0.13

CI=3.25±0.36)were unreached to positive response standard

corresponding change was not obvious. Conclusion: ZJF

QKL and CXL have the allergenic potential the sensitization of CH or BLG is not obvious.